[Inside front cover] In Situ Biosynthesis of a Metal Nanoparticle Encapsulated in Alginate Gel for Imageable Drug-Delivery Syste
Title :In Situ Biosynthesis of a Metal Nanoparticle Encapsulated in Alginate Gel for Imageable Drug-Delivery System
Author : Moon Il Kim, Chan Yeong Park, Ji Min Seo, Kyoung Suk Kang, Ki Soo Park, Jongeun Kang, Kwan Soo Hong,
Yoojin Choi, Sang Yup Lee, Jong Pil Park, Hyun Gyu Park, and Tae Jung Park
Journal :ACS Applied Materials and Interfaces
Abastract : Development of drug-delivery systems that allow simultaneous in vivo imaging has gained much interest.
We report a novel strategy to encapsulate metal nanoparticles (NPs) within alginate gel for in vivo imaging.
The cell lysate of recombinant Escherichia coli strain, expressing Arabidopsis thaliana phytochelatin synthase
and Pseudomonas putida metallothionein genes, was encapsulated within the alginate gel. Incubation of alginate gel
with metal ion precursors followed by UV irradiation resulted in the synthesis of high concentrations of metal NPs, such as Au, Ag, CdSe,
and EuSe NPs, within the gel.
The alginate gel with metal NPs was used as a drug-delivery system by further co-encapsulating doxorubicin and rifampicin,
the release of which was made to be pH-dependent. This system can be conveniently and safely used for in vitro and in vivo bioimaging,
enabled by the metal NPs formed within the gel matrix without using toxic reducing reagents or surfactants.