•  [Outside back cover] CRISPR/Cas12a collateral cleavage activity for an ultrasensitive assay of RNase H 
  • 관리자 |
  • 2022-03-02 16:52:57|
  • 569

Title: CRISPR/Cas12a collateral cleavage activity for an ultrasensitive assay of RNase H 


Author: Hansol Kim, Seoyoung Lee, Jinhwan Lee and Hyun Gyu Park 


Journal: Chemical communications, 2022, 58, 2654-2657


Abstract : We herein describe an ultrasensitive RNase H assay by utilizing CRISPR/Cas12a collateral cleavage activity. Based on this unique design principle,


the RNase H activity was successfully determined down to 0.00024 U mL−1, which is quite superior to those of alternative approaches.




   
첨부파일