• High-titer, antibiotic-free, pilot-scale production of 1,3-propanediol by engineered Corynebacterium
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  • 2026-05-19 14:01:20|
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ㅇ [Title]         High-titer, antibiotic-free, pilot-scale production of 1,3-propanediol by engineered Corynebacterium

  

ㅇ [Journal]    Nature Chemical Engineering


                                                                         
ㅇ [Author]     Jae Sung Cho *, Cindy Pricilia Surya Prabowo*, Taehee Han, Cheon Woo Moon, Yoo-Sung Ko, Changhee Cho, Je Woong Kim, Won Jun Kim, Hyun Bae Bang,
                   Jae Eun Lee, Minjung Ki, Namjin Jang & Sang Yup Lee
                      *co-first author




 


ㅇ [Abstrct]


The microbial valorization of glycerol, a major biodiesel byproduct, into high-value chemicals remains challenging at industrially competitive titers. Here we engineered

Corynebacterium glutamicum for high-level 1,3-propanediol (1,3-PDO) production, validating each step via fed-batch fermentation. First, C. glutamicum ATCC 13032 was

metabolically engineered to produce 138 g l−1 1,3-PDO from glucose–glycerol and 100.9 g l−1 from glycerol alone. Key engineering strategies included establishing glycerol

uptake and 1,3-PDO biosynthetic pathways, minimizing byproducts and optimizing fed-batch fermentation. We then transferred these strategies to a newly isolated strain,

C. glutamicum SC97. Further engineering,including antibiotic-free plasmid addiction system and sucCD overexpression, enabled 141.5 g l−1 1,3-PDO at 2.95 g l−1 h−1

without antibiotics. Scalability was demonstrated at 30-l and 300-l pilot-scale fermentations, reaching 120.2 g l−1 and 127.8 g l−1 of 1,3-PDO, respectively. Techno-

economic and life-cycle assessments support industrial feasibility and environmental impact, providing a robust blueprint for sustainable microbial 1,3-PDO production

at scale.





 






 
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