• Gram-per-litre-scale production of lutein by engineered Corynebacterium
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  • 2025-07-14 11:13:20|
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ㅇ [Title]        Gram-per-litre-scale production of lutein by engineered Corynebacterium

 
ㅇ [Journal]  Nature Synthesis
                                                                                                                                                                                                        

ㅇ [Author]   Hyunmin Eun, Cindy Pricilia Surya Prabowo & Sang Yup Lee


ㅇ [Abstrct]


Lutein is commonly used as a supplement for ocular health. However, commercial lutein is mainly extracted from marigold flowers, an inefficient process that includes secondary metabolites. Here, building on our

previous research on lutein synthesis in engineered Escherichia coli, we report the development of a microbial platform for efficient lutein production using metabolically engineered Corynebacterium glutamicum.

We engineered C. glutamicum to produce lycopene and introduced the lutein biosynthesis pathway. Then, we substantially enhanced lutein production by optimizing the haem pathway, engineering the P450 reductase

and utilizing an optimal electron-channelling scaffold system to improve electron transfer reactions, addressing the rate-limiting steps in lutein synthesis. This approach achieved a substantial increase in

lutein production, reaching a concentration of 1.78 g l−1, with a content of 19.51  mg  per gram dry cell weight and a productivity of 32.88 mg l−1 h−1 in fed-batch fermentation. This approach allows gram-per-litre

-scale microbial production of lutein.

 
 
Eun, H., Prabowo, C.P.S. & Lee, S.Y. Gram-per-litre-scale production of lutein by engineered Corynebacterium. Nat. Synth (2025). https://doi.org/10.1038/s44160-025-00826-3



 

 
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