Department of Chemical and Biomolecular Engineering
Korea Advanced Institute of Science and Technology

Recent Achievements

제목

[Inside front cover]SERS‐Active‐Charged Microgels for Size‐ and Charge‐Selective Molecular Analysis of Complex Biological Samples

Title: SERSActiveCharged Microgels for Size and ChargeSelective Molecular Analysis of Complex Biological Samples

Author: Dong Jae Kim, SungGyu Park, DongHo Kim,* and ShinHyun Kim*

Journal: Small, 2018, 14, 1802520

Abstract: Surfaceenhanced Raman scattering (SERS) provides a dramatic increase of Raman intensity for molecules adsorbed on nanogaprich metal nanostructures, serving as a promising tool for molecular analysis. However, surface contamination caused by protein adsorption and low surface concentration of small target molecules reduce the sensitivity, which severely restricts the use of SERS in many applications. Here, charged microgels containing agglomerates of gold nanoparticles (Au NPs) are designed using dropletbased microfluidics to provide a reliable SERS substrate with molecular selectivity and high sensitivity. The limiting mesh size of hydrogel enables the autonomous exclusion of large proteins and the charged matrix concentrates oppositely charged small molecules through electrostatic attraction. As nanogaps among Au NPs in the agglomerates enhance Raman intensity, Raman spectrum of the adsorbed molecules is selectively measured with high sensitivity in the absence of interruption from adhesive proteins. Therefore, the SERSactivecharged microgels can be used for direct analysis of pristine biological samples without the pretreatment steps of separation and concentration, which are commonly a prerequisite for Raman analysis. For the purpose of demonstration, a direct detection of fipronil sulfone with partial negative charges, a metabolite of toxic insecticide, dissolved in eggs using the positively charged microgels without any pretreatment of the samples, is shown. 

 

 

 

첨부파일 다운로드

등록자관리자

등록일2018-11-08

조회수3,318

  • 페이스북 공유
  • 트위터 공유
  • Google+ 공유
  • 인쇄하기
 
사진제목
Sustainable Redox Mediation for Lithium–Oxygen Batteries by a Composite Protective Layer on the Lithium-Metal Anode   Dong Jin Lee†, Hongkyung Lee†, Yun-Jung Kim , Jung-Ki Park ,* and Hee-Tak Kim* († denotes equal contribution)   Advanced Materials, Vol. 28, Issue 5, page 857-863 (February 3, 2016) [Abstract]A synergic combination of a soluble &sh...
Liquid-Impermeable Inverse Opals with Invariant Photonic Bandgap (pages 1282–1287) Hyelim Kang, Joon-Seok Lee, Won Seok Chang and Shin-Hyun Kim Article first published online: 10 DEC 2014 | DOI: 10.1002/adma.201404706 [ABSTRACT] Omniphobic inverse opals are created by structurally and chemically modifying the surface of inverse opals through reactive ion etching. During the etching, ...
Robust Microfluidic Encapsulation of Cholesteric Liquid Crystals toward Photonic Ink Capsules,` Advanced Materials, 27, 627−633 (2015) Sang Seok Lee, Bomi Kim, Su Kyung Kim, Jong Chan Won, Yun Ho Kim, and Shin-Hyun Kim 관련기사:  http://www.hellodd.com/news/article.html?no=51837  [ABSTACT] Robust photonic microcapsules are created by microfluidic encapsulation of chol...
Cofactor-Free Light-Driven Whole-Cell Cytochrome P450 Catalysis Angew. Chem. Int. Ed. 54(3):969-973 (Jan. 12 2015)   Park JH, Lee SH, Cha GS, Choi DS, Nam DH, Lee JH, Lee JK, Yun CH, Jeong KJ*, Park, CB*  [ABSTRACT] Cytochromes P450 can catalyze various regioselective and stereospecific oxidation reactions of non-functionalized hydrocarbons. Here, we have designed a novel ...
Metabolic engineering of antibiotic factories: new tools for antibiotic production in actinomycetes Tilmann Weber, Pep Charusanti, Ewa Maria Musiol-Kroll, Xinglin Jiang, Yaojun Tong, Hyun Uk Kim, Sang Yup Lee  [ABSTRACT] Actinomycetes contain diverse antibiotic gene clusters that have not been fully explored yet. Existing and recently developed tools now allow us to perform metabolic ...