Department of Chemical and Biomolecular Engineering
Korea Advanced Institute of Science and Technology

Recent Achievements

제목

[Inside front cover]SERS‐Active‐Charged Microgels for Size‐ and Charge‐Selective Molecular Analysis of Complex Biological Samples

Title: SERSActiveCharged Microgels for Size and ChargeSelective Molecular Analysis of Complex Biological Samples

Author: Dong Jae Kim, SungGyu Park, DongHo Kim,* and ShinHyun Kim*

Journal: Small, 2018, 14, 1802520

Abstract: Surfaceenhanced Raman scattering (SERS) provides a dramatic increase of Raman intensity for molecules adsorbed on nanogaprich metal nanostructures, serving as a promising tool for molecular analysis. However, surface contamination caused by protein adsorption and low surface concentration of small target molecules reduce the sensitivity, which severely restricts the use of SERS in many applications. Here, charged microgels containing agglomerates of gold nanoparticles (Au NPs) are designed using dropletbased microfluidics to provide a reliable SERS substrate with molecular selectivity and high sensitivity. The limiting mesh size of hydrogel enables the autonomous exclusion of large proteins and the charged matrix concentrates oppositely charged small molecules through electrostatic attraction. As nanogaps among Au NPs in the agglomerates enhance Raman intensity, Raman spectrum of the adsorbed molecules is selectively measured with high sensitivity in the absence of interruption from adhesive proteins. Therefore, the SERSactivecharged microgels can be used for direct analysis of pristine biological samples without the pretreatment steps of separation and concentration, which are commonly a prerequisite for Raman analysis. For the purpose of demonstration, a direct detection of fipronil sulfone with partial negative charges, a metabolite of toxic insecticide, dissolved in eggs using the positively charged microgels without any pretreatment of the samples, is shown. 

 

 

 

0

추천하기

0

반대하기

첨부파일 다운로드

등록자관리자

등록일2018-11-08

조회수1,473

  • 페이스북 공유
  • 트위터 공유
  • Google+ 공유
  • 인쇄하기
 
스팸방지코드 :
사진제목
Ki Soo Park, Cheulhee Jung and Hyun Gyu Park Angewandte Chemie International Edition, 49(50), 9757-9760, (2010.12.10) Article first published online: 26 OCT 2010 DOI: 10.1002/anie.201004406 http://onlinelibrary.wiley.com/doi/10.1002/anie.201004406/abstract Abstract Metal ion, an astonishing illusionist: In a new concept, “illusionary” polymerase activity is intentionally...