Department of Chemical and Biomolecular Engineering
Korea Advanced Institute of Science and Technology

Recent Achievements

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[Front Cover]New Platform for cytochrome P450 reaction combining in situ immobilization on biopolymer

New Platform for cytochrome P450 reaction combining in situ immobilization on biopolymer.

 

Lee JH, Nam DH, Lee SH, Park JH, Park SJ, Lee SH, Park CB, Jeong KJ

 

Bioconjugate Chemistry (2014) 25(12):2101-2104.

 

[ABSTRACT]We describe an efficienct chemical conversion platform with in situ immobilization of P450-BM3 on poly(3-hydroxybutyrate) granules. Through fusion with phasin, P450-BM3 is easily immobilized on poly(3-hydroxybutyrate) granules in Escherichia coli. In our work, the immobilized P450 exhibited higher stability and catalytic activity compared to free P450 against changes of pH, temperature, and concentrations of urea and ions. Through quick recovery of immobilized enzyme, the P450–P(3HB) complex successfully catalyzed an O-dealkylation reaction several times with maintained activity. Using the robust P450–P(3HB) complex, we performed a P450-catalyzed reaction on a preparative reactor scale (100 mL) and high-level production (12.3 μM) of 7-hydroxycoumarine from 7-ethoxycoumarin could be achieved.


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등록자배성순

등록일2014-12-23

조회수6,479

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사진제목
Controlling Molecular Orientation of Naphthalenediimide-Based Polymer Acceptors for HighPerformance All-Polymer Solar Cells Author: Jihye Jung, Wonho Lee, Changyeon Lee, Hyungju Ahn, Bumjoon J.Kim Journal: Advanced Energy Materials DOI: 10.1002/aen,.201600504 [ABSTRACT]Molecular orientation, with respect to donor/acceptor interface andelectrodes, plays a critical role in det...
A Highly Sensitive Molecular Detection Platform for Robust and Facile Diagnosis of Middle East Respiratory Syndrome (MERS) Corona VirusAuthor : Il Young Jung, Jae Bem You, Bo Ram Choi, Ji Su Kim, Hyun Kyung Lee, Bora Jang, Han Saem Jeong, Kyuri Lee, Sung Gap Im*, Hyukjin Lee*Journal : Advanced Healthcare Materials 2016, 5, 2168-2173[ABSTRACT]In this work, we demonstrate a definitive ...
Size-controlled gold nano-tetradecapodswith tunable optical and electromagnetic propertiesAuthor : Kyeong Woo Choi, Shin Wook Kang, Do Youb Kim*, Sang Hyuk Im, Yangsun Park, Sang Woo Han* and O Ok Park*Journal : Journal of Materials Chemistry CPage : Vol. 4, No. 15, page 3063-3350(April, 2016)[ABSTRACT]The synthesis of novel branched Aunanocrystals with a well-defined and size-controllable dualist...
Title: Metal ion triggers for reversible switching of DNA polymeraseAuthors: Ki Soo Park, Chang Yeol Lee, and Hyun Gyu Park Journal: Chemical CommunicationsPage: Vol. 52, page 4868-4871 (April, 2016) [Abstract] We herein develop a new strategy that modulates DNA polymerase activity in a reversible and switchable manner by using the novel interactions between DNA bases and metal...
Tuning selectivity of electrochemical reactions by atoomically dispersed platinum catalyst Chang Hyuck Choi, Minho Kim, Han Chang Kwon, Sung June Cho, Seongho Yun, Hee-Tak Kim, Karl J.J. Mayrhofer, Hyungjun Kim, Minkee Choi Nature Communications, Vol. 7, page 10922 (March 8, 2016) [Abstract] Maximum atom efficiency as well as distinct chemoselectivity is expected for electrocat...