Department of Chemical and Biomolecular Engineering
Korea Advanced Institute of Science and Technology

Recent Achievements

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[Front Cover]New Platform for cytochrome P450 reaction combining in situ immobilization on biopolymer

New Platform for cytochrome P450 reaction combining in situ immobilization on biopolymer.

 

Lee JH, Nam DH, Lee SH, Park JH, Park SJ, Lee SH, Park CB, Jeong KJ

 

Bioconjugate Chemistry (2014) 25(12):2101-2104.

 

[ABSTRACT]We describe an efficienct chemical conversion platform with in situ immobilization of P450-BM3 on poly(3-hydroxybutyrate) granules. Through fusion with phasin, P450-BM3 is easily immobilized on poly(3-hydroxybutyrate) granules in Escherichia coli. In our work, the immobilized P450 exhibited higher stability and catalytic activity compared to free P450 against changes of pH, temperature, and concentrations of urea and ions. Through quick recovery of immobilized enzyme, the P450–P(3HB) complex successfully catalyzed an O-dealkylation reaction several times with maintained activity. Using the robust P450–P(3HB) complex, we performed a P450-catalyzed reaction on a preparative reactor scale (100 mL) and high-level production (12.3 μM) of 7-hydroxycoumarine from 7-ethoxycoumarin could be achieved.


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등록자배성순

등록일2014-12-23

조회수6,461

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사진제목
Title: Polyketide bio-derivatization using the promiscuous acyltransferase KirCII Author: Musiol-Kroll, E.M., Zubeil, F., Schafhauser, T., Hartner, T., Kulik, A., McArthur, J., Koryakina, I., Wohlleben, W., Grond, S., Williams, G.J., Lee, S.Y., and Weber, T. Journal: ACS Synth. Biol., 2017, 6 (3), pp 421–427   Abstract: During polyketide bios...
Title: Structure and function of the N-terminal domain of Ralstonia eutropha polyhydroxyalkanoate synthase, and the proposed structure and mechanisms of the whole enzyme Author: Kim, Y-J., Choi, S.Y., Kim, J.E., Jin, K.S., Lee, S.Y., Kim, K-J. Journal: Biotechnol. J., 12(1):  1-12 (2017. 1) Abstract: Polyhydroxyalkanoates (PHAs) are natural...
Title: Crystal structure of Ralstonia eutrophapolyhydroxyalkanoate synthase C-terminal domain and reaction mechanismsAuthor: Kim, J.E., Kim, Y-J., Choi, S.Y., Lee, S.Y.,Kim, K-J.Journal: Biotechnol. J., 12(1):  1-12(2017. 1)Abstract:Polyhydroxyalkanoates(PHAs) are natural polyesters synthesized by numerous microorganisms as energyand reducing power storage materials, and h...
Title: Biosynthesis of poly(2-hydroxyisovalerate-co-lactate) by metabolically engineered Escherichia coli   Author: Yang, J.E., Kim, J.W., Oh, Y.H., Choi, S.Y., Lee, H., Park, A.-R., Shin, J., Park, S.J., and Lee, S.Y.  Journal: Biotechnol. J. , 11(12): 1572-1585 (2016. 12)  Abstract: Polyhydroxyalkanoates (PHAs) containing 2-hydroxy...
Title: Fluorescent Block Copolymer-MoS2Nanocomposites for Real-Time Photothermal Heating and Imaging Author: Chan Ho Park, Hongseok Yun, Hyunseung Yang, JunhyukLee, and Bumjoon J. Kim* Journal: Advanced Functional Materials, 2017, 27, 1604403 Abstract:Novel self-monitoring photothermal (PT) agents are developed using optothermally responsive block copolymer-MoS2 ...