Department of Chemical and Biomolecular Engineering
Korea Advanced Institute of Science and Technology

Recent Achievements

제목

[Front Cover]New Platform for cytochrome P450 reaction combining in situ immobilization on biopolymer

New Platform for cytochrome P450 reaction combining in situ immobilization on biopolymer.

 

Lee JH, Nam DH, Lee SH, Park JH, Park SJ, Lee SH, Park CB, Jeong KJ

 

Bioconjugate Chemistry (2014) 25(12):2101-2104.

 

[ABSTRACT]We describe an efficienct chemical conversion platform with in situ immobilization of P450-BM3 on poly(3-hydroxybutyrate) granules. Through fusion with phasin, P450-BM3 is easily immobilized on poly(3-hydroxybutyrate) granules in Escherichia coli. In our work, the immobilized P450 exhibited higher stability and catalytic activity compared to free P450 against changes of pH, temperature, and concentrations of urea and ions. Through quick recovery of immobilized enzyme, the P450–P(3HB) complex successfully catalyzed an O-dealkylation reaction several times with maintained activity. Using the robust P450–P(3HB) complex, we performed a P450-catalyzed reaction on a preparative reactor scale (100 mL) and high-level production (12.3 μM) of 7-hydroxycoumarine from 7-ethoxycoumarin could be achieved.


첨부파일 다운로드

등록자배성순

등록일2014-12-23

조회수6,457

  • 페이스북 공유
  • 트위터 공유
  • Google+ 공유
  • 인쇄하기
 
사진제목
Title: Stimuli-Responsive, Shape-Transforming Nanostructured Particles Author: Junhyuk Lee, Kang Hee Ku, Mingoo Kim, Jae Man Shin, Junghun Han, Chan Ho Park, Gi-Ra Yi, Se Gyu Jang, Bumjoon J. Kim*   Journal: Advanced Materials, 2017, Vol.29, 1700608 Abstract Dynamic, temperature-driven transformation of the shap...
Title:  [Frontispiece] Structural Color Palettes of Core-Shell Photonic InkCapsules containing Cholesteric Liquid CrystalsAuthor: Sang Seok Lee, HyeonJin Seo, Yun Ho Kim,* and Shin-Hyun Kim*Journal: AdvancedMaterials, 2017, Vol.29, 1606894AbstractPhotonicmicrocapsules with onion-like topology are microfluidically designed to have cholesteric liquid crystals with oppositehandedness in th...
Title:  [Front cover] Selective Coloration of Melanin Nanospheres throughResonant Mie ScatteringAuthor: Soojeong Cho, Tae SoupShim, Ju Hyeon Kim, Dong-Hyun Kim, and Shin-Hyun Kim*Journal: Advanced Materials,2017, Vol.29, 1700256AbstractBlack melanin inks are preparedto selectively exhibit colors under strong light, inspired by human hair. Highabsorbance of melanin suppresses multiple sca...
Title:  [Inside Front Cover] An Ultrasensitive, Visco-Poroelastic Artificial Mechanotransducer Skin Inspired by Piezo2 Protein in Mammalian Merkel Cells  Author: Ming Liang Jin†, Sangsik Park†, Younghoon Lee, Ji Hye Lee, Junho Chung, Joo Sung Kim, Jong-Seon Kim, So Young Kim, Eunsong Jee, Dae Woo Kim, Jae Woo Chung,Seung Geol Lee, Dukhyun Choi, Hee-Tae Jung* ...
Title:  A simple and eco-friendly one-pot synthesis of nuclease-resistant DNA-inorganic hybrid nanoflowers   Author: Ki Soo Park, Bhagwan S Batule, Minsoo Chung, Kyoung Suk Kang, Tae Jung Park, Moon Il Kim and Hyun Gyu Park*   Journal: Journal of Material Chemistry B, 2017, 5, 2231-2234   Abstract: A simple and eco-friendly method has b...