Department of Chemical and Biomolecular Engineering
Korea Advanced Institute of Science and Technology

Recent Achievements

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[Front Cover]New Platform for cytochrome P450 reaction combining in situ immobilization on biopolymer

New Platform for cytochrome P450 reaction combining in situ immobilization on biopolymer.

 

Lee JH, Nam DH, Lee SH, Park JH, Park SJ, Lee SH, Park CB, Jeong KJ

 

Bioconjugate Chemistry (2014) 25(12):2101-2104.

 

[ABSTRACT]We describe an efficienct chemical conversion platform with in situ immobilization of P450-BM3 on poly(3-hydroxybutyrate) granules. Through fusion with phasin, P450-BM3 is easily immobilized on poly(3-hydroxybutyrate) granules in Escherichia coli. In our work, the immobilized P450 exhibited higher stability and catalytic activity compared to free P450 against changes of pH, temperature, and concentrations of urea and ions. Through quick recovery of immobilized enzyme, the P450–P(3HB) complex successfully catalyzed an O-dealkylation reaction several times with maintained activity. Using the robust P450–P(3HB) complex, we performed a P450-catalyzed reaction on a preparative reactor scale (100 mL) and high-level production (12.3 μM) of 7-hydroxycoumarine from 7-ethoxycoumarin could be achieved.


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등록자배성순

등록일2014-12-23

조회수6,482

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사진제목
Title: Fully Dispersed Rh Ensemble Catalyst To Enhance Low-Temperature Activity   Author: Hojin Jeong, Geonhee Lee, Beom-Sik Kim, Junemin Bae, Jeong Woo Han, and Hyunjoo Lee*   Journal: Journal of American Chemical Society, 2018, 140, 9558-9565   Abstract: Minimizing the use of precious metal catalysts is important in many applicati...
Title: Domain Structures of Poly(3-dodecylthiophene)-Based Block Copolymers Depend onRegioregularity Author: Jin-Seong Kim, Junghun Han, Youngkwon Kim, Hyeonjung Park,Jonathan P. Coote Gila E Stein*, and Bumjoon J. Kim* Journal: Macromolecules 51, 11, 4077-4084, Outside frontcover Abstract: Microphase-separation behavior of conjugated–amorphous block copol...
Title: An Ultrastable Ionic Chemiresistor Skin with an Intrinsically Stretchable Polymer ElectrolyteAuthor: Ming Liang Jin, Sangsik Park, Jong-Seon Kim, Sung Hyun Kwon, Shuye Zhang, Min Seok Yoo, Sungwoo Jang, Hyeong-Jun Koh, Soo-Yeon Cho, So Young Kim, Chi Won Ahn, Kilwon Cho, Seung Geol Lee, Do Hwan Kim,* and Hee-Tae Jung*Journal: Advanced Materials, 2018, Vol.30, 1706851Abstract: Ultrastable se...
Title: Cancer Treatment: Enhanced Photodynamic Cancer Treatment by Mitochondria‐Targeting and Brominated Near‐Infrared Fluorophores Author: Ilkoo Noh, DaeYong Lee, Heegon Kim, Chan-Uk Jeong, Yunsoo Lee, Jung-Oh Ahn, Hoon Hyun, Ji-Ho Park, and Yeu-Chun Kim Journal: Advanced science (IF:9.034) Abstract: A noninvasive and selective therapy, photodynamic ther...
Title: Metallic Ti3C2Tx MXene Gas Sensors with Ultrahigh Signal-to-Noise Ratio Author: Seon Joon Kim, Hyeong-Jun Koh, Chang E. Ren, Ohmin Kwon, Kathleen Maleski, Soo-Yeon Cho, Babak Anasori, Choong-Ki Kim, Yang-Kyu Choi, Jihan Kim, Yury Gogotsi*, Hee-Tae Jung* Journal: ACS Nano 2018, 12 (2), 986-993. Abstract: Achieving high...