Department of Chemical and Biomolecular Engineering
Korea Advanced Institute of Science and Technology

Recent Achievements

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[Front Cover]New Platform for cytochrome P450 reaction combining in situ immobilization on biopolymer

New Platform for cytochrome P450 reaction combining in situ immobilization on biopolymer.

 

Lee JH, Nam DH, Lee SH, Park JH, Park SJ, Lee SH, Park CB, Jeong KJ

 

Bioconjugate Chemistry (2014) 25(12):2101-2104.

 

[ABSTRACT]We describe an efficienct chemical conversion platform with in situ immobilization of P450-BM3 on poly(3-hydroxybutyrate) granules. Through fusion with phasin, P450-BM3 is easily immobilized on poly(3-hydroxybutyrate) granules in Escherichia coli. In our work, the immobilized P450 exhibited higher stability and catalytic activity compared to free P450 against changes of pH, temperature, and concentrations of urea and ions. Through quick recovery of immobilized enzyme, the P450–P(3HB) complex successfully catalyzed an O-dealkylation reaction several times with maintained activity. Using the robust P450–P(3HB) complex, we performed a P450-catalyzed reaction on a preparative reactor scale (100 mL) and high-level production (12.3 μM) of 7-hydroxycoumarine from 7-ethoxycoumarin could be achieved.


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등록자배성순

등록일2014-12-23

조회수6,483

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사진제목
Ki Soo Park, Cheulhee Jung and Hyun Gyu Park Angewandte Chemie International Edition, 49(50), 9757-9760, (2010.12.10) Article first published online: 26 OCT 2010 DOI: 10.1002/anie.201004406 http://onlinelibrary.wiley.com/doi/10.1002/anie.201004406/abstract Abstract Metal ion, an astonishing illusionist: In a new concept, “illusionary” polymerase activity is intentionally...