Department of Chemical and Biomolecular Engineering
Korea Advanced Institute of Science and Technology

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[Front Cover]New Platform for cytochrome P450 reaction combining in situ immobilization on biopolymer

New Platform for cytochrome P450 reaction combining in situ immobilization on biopolymer.

 

Lee JH, Nam DH, Lee SH, Park JH, Park SJ, Lee SH, Park CB, Jeong KJ

 

Bioconjugate Chemistry (2014) 25(12):2101-2104.

 

[ABSTRACT]We describe an efficienct chemical conversion platform with in situ immobilization of P450-BM3 on poly(3-hydroxybutyrate) granules. Through fusion with phasin, P450-BM3 is easily immobilized on poly(3-hydroxybutyrate) granules in Escherichia coli. In our work, the immobilized P450 exhibited higher stability and catalytic activity compared to free P450 against changes of pH, temperature, and concentrations of urea and ions. Through quick recovery of immobilized enzyme, the P450–P(3HB) complex successfully catalyzed an O-dealkylation reaction several times with maintained activity. Using the robust P450–P(3HB) complex, we performed a P450-catalyzed reaction on a preparative reactor scale (100 mL) and high-level production (12.3 μM) of 7-hydroxycoumarine from 7-ethoxycoumarin could be achieved.


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등록자배성순

등록일2014-12-23

조회수6,454

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사진제목
Hierarchical Structures: 3D Hierarchical Architectures Prepared by Single Exposure Through a Highly Durable Colloidal Phase Mask (Adv. Mater. 9/2014) Tae Yoon Jeon, Hwan Chul Jeon, Su Yeon Lee, Tae Soup Shim, Jung-Dae Kwon, Sung-Gyu Park, Seung-Man Yang Article first published online: 4 MAR 2014 DOI: 10.1002/adma.201470058 [ABSTRACT] A simple and cost-effective fabrication of 3...
Droplet Microfluidics for Producing Functional Microparticles  Ju Hyeon Kim, Tae Yoon Jeon, Tae Min Choi, Tae Soup Shim, Shin-Hyun Kim, and Seung-Man Yang [ABSTRACT] Isotropic microparticles prepared from a suspension that undergoes polymerization have long been used for a variety ofapplications. Bulk emulsification procedures produce polydisperse emulsion droplets that are transfor...
Tuning Cage Dimension in Clathrate Hydrates for Hydrogen Multiple Occupancy Dong-Yeun Koh †, Hyery Kang †, Jiwon Jeon ‡, Yun-Ho Ahn †, Youngjune Park †, Hyungjun Kim *‡, and Huen Lee *†‡ †Department of Chemical and Biomolecular Enginieering and ‡Graduate School of EEWS, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daej...
Microbial production of short-chain alkanes Yong Jun Choi & Sang Yup Lee Nature Volume:502,Pages:571–574Date published:(24 October 2013)DOI:doi:10.1038/nature12536 Increasing concerns about limited fossil fuels and global environmental problems have focused attention on the need to develop sustainable biofuels from renewable resources. Although microbial production of diesel...
Title: Synthesis of a 3D graphite microball using a microfluidic droplet generator and its polymer composite with core–shell structure Author: Dong Ju Han, Jae Hwan Jung, Jong Seob Choi, Yong Tae Kim and Tae Seok Seo Journal : Lab on a Chip 2013,13, 4006 Lab Chip, 2013,13, 4006-4010 DOI: 10.1039/C3LC50838B ABSTRACT Spherical 3D graphite microballs (3D GMs) and their nanohybri...