Department of Chemical and Biomolecular Engineering
Korea Advanced Institute of Science and Technology

Recent Achievements

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[Front Cover]New Platform for cytochrome P450 reaction combining in situ immobilization on biopolymer

New Platform for cytochrome P450 reaction combining in situ immobilization on biopolymer.

 

Lee JH, Nam DH, Lee SH, Park JH, Park SJ, Lee SH, Park CB, Jeong KJ

 

Bioconjugate Chemistry (2014) 25(12):2101-2104.

 

[ABSTRACT]We describe an efficienct chemical conversion platform with in situ immobilization of P450-BM3 on poly(3-hydroxybutyrate) granules. Through fusion with phasin, P450-BM3 is easily immobilized on poly(3-hydroxybutyrate) granules in Escherichia coli. In our work, the immobilized P450 exhibited higher stability and catalytic activity compared to free P450 against changes of pH, temperature, and concentrations of urea and ions. Through quick recovery of immobilized enzyme, the P450–P(3HB) complex successfully catalyzed an O-dealkylation reaction several times with maintained activity. Using the robust P450–P(3HB) complex, we performed a P450-catalyzed reaction on a preparative reactor scale (100 mL) and high-level production (12.3 μM) of 7-hydroxycoumarine from 7-ethoxycoumarin could be achieved.


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등록자배성순

등록일2014-12-23

조회수6,612

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[inside front cover] Hye Soo Lee, Ju Hyeon Kim, Joon-Seok Lee, Jae Young Sim, Jung Yoon Seo, You-Kwan Oh, Seung-Man Yang, and Shin-Hyun Kim*, `Magnetoresponsive Discoidal Photonic Crystals towards Active Color Pigments,` Advanced Materials, 26, 5801-5807 (2014). [ABSTACT] Morpho butterflys show beautiful colors that arise from periodic nanostructures. Inspired by the butterfly, colloida...
Site-specific immobilization of proteins on non-conventional substrates via solvent-free initiated chemical vapor deposition (iCVD) process. Jeong, G.M., Seong, H., Kim, Y.S., Im, S.G., Jeong, K.J.   Polymer Chemistry 5:4459-4465 (2014)   [ABSTRACT] A simple protein-immobilization method on various substrates, including paper and polyethylene film, was developed using...
Highly sensitive detection of cancer cells based on the DNA barcode assay and microcapillary electrophoretic analysis Soyi Chung, Minkyung Cho, Jae Whan Jung and Tae Seok Seo Article first published online: 8 MAY 2014 DOI: 10.1002/elps.201470091 [ABSTRACT] Combination of DNA biobarcode assay with micro-capillary electrophoretic analysis on a chip allows us to perform breast and ...
Light-Emitting Diodes: Simultaneously Enhancing Light Extraction and Device Stability of Organic Light-Emitting Diodes using a Corrugated Polymer Nanosphere Templated PEDOT:PSS Layer (Adv. Energy Mater. 8/2014) Changyeon Lee, Dong Jin Kang, Hyunbum Kang, Taesu Kim, Junwoo Park, Jaeho Lee, Seunghyup Yoo and Bumjoon J. Kim Article first published online: 3 JUN 2014 | DOI: 10.1002/aenm.2014...
Microcapsules: Osmocapsules for Direct Measurement of Osmotic Strength (Small 6/2014) (page 1232) Shin-Hyun Kim, Tae Yong Lee and Sang Seok Lee Article first published online: 24 MAR 2014 | DOI: 10.1002/smll.201470037 [ABSTRACT] Osmocapsules are microfluidically created to provide a tool for direct measurement of osmotic pressure. As reported by S.-H. Kim and co-workers on page 1155,...