Department of Chemical and Biomolecular Engineering
Korea Advanced Institute of Science and Technology

Recent Achievements

제목

[Cover Page]Rational Design of Escherichia coli for l-Isoleucine Production

Rational Design of Escherichia coli for l-Isoleucine Production

Jin Hwan Park , Jae Eun Oh , Kwang Ho Lee , Ji Young Kim , and Sang Yup Lee

 

ABSTRACT  

Metabolic engineering of Escherichia coli was performed to construct a 100% rationally engineered strain capable of overproducing l-isoleucine, an important branched-chain amino acid. The thrABC (encoding l-threonine biosynthetic enzymes), ilvA (encoding feedback-resistant threonine dehydratase), ilvIH (encoding feedback-resistant acetohydroxy acid synthase III), and ygaZH (encoding branched-chain amino acid exporter) genes were amplified by plasmid-based overexpression. The ilvCED (encoding l-isoleucine biosynthetic enzymes) and lrp (encoding global regulator Lrp) genes were also amplified by chromosomal promoter replacement in order to further increase the flux toward l-isoleucine. The final engineered E. coli strain was able to produce 9.46 g/L of l-isoleucine with a yield of 0.14 g/g of glucose by fed-batch culture. The overall design principles described here for the production of highly regulated product should be useful in designing strains for the production of other similar bioproducts.  

 

첨부파일 다운로드

등록자배성순

등록일2012-11-30

조회수9,847

  • 페이스북 공유
  • 트위터 공유
  • Google+ 공유
  • 인쇄하기
 
사진제목
Ki Soo Park, Cheulhee Jung and Hyun Gyu Park Angewandte Chemie International Edition, 49(50), 9757-9760, (2010.12.10) Article first published online: 26 OCT 2010 DOI: 10.1002/anie.201004406 http://onlinelibrary.wiley.com/doi/10.1002/anie.201004406/abstract Abstract Metal ion, an astonishing illusionist: In a new concept, “illusionary” polymerase activity is intentionally...