Department of Chemical and Biomolecular Engineering
Korea Advanced Institute of Science and Technology

Recent Achievements

제목

[outside front cover] A label-free and enzyme-free signal amplification strategy for a sensitive RNase H activity assay

Title: A label-free and enzyme-free signal amplification strategy for a sensitive RNase H activity assay 

 

Author: Chang Yeol Lee, Hyowon Jang,Ki Soo Park* and Hyun Gyu Park* (: equal contribution) 

 

Journal:  Nanoscale 2017, 9, 16149-16153


Abstract: We herein describe a label-free and enzyme-free signal amplification strategy for the sensitive determination of ribonuclease H (RNase H) activity, which relies on the target-triggered catalytic hairpin assembly (CHA) in conjunction with a G-quadruplex specific fluorescent binder, N-methyl mesoporphyrin IX (NMM). In the absence of RNase H, the RNA/DNA duplex serving as a substrate for RNase H cannot initiate the execution of CHA that produces G-quadruplexes; so NMM shows a low fluorescence signal. In contrast, the presence of RNase H that degrades RNA in the RNA/DNA duplex releases DNA designed to function as the catalyst for CHA. This consequently promotes the efficient CHA and generates a large number of G-quadruplexes with a significantly enhanced fluorescence signal from NMM. Based on this label-free and enzyme-free signal amplification strategy, we successfully determined the RNase H activity with a detection limit of 0.037 U mL1 and screened potential RNase H inhibitors. Our results suggest that the developed system is a promising platform for a cost-effective, sensitive enzyme activity assay and inhibitor screening. 

 



 

 

0

추천하기

0

반대하기

첨부파일 다운로드

등록자관리자

등록일2017-11-06

조회수3,921

  • 페이스북 공유
  • 트위터 공유
  • Google+ 공유
  • 인쇄하기
 
스팸방지코드 :
사진제목
Liquid-Impermeable Inverse Opals with Invariant Photonic Bandgap (pages 1282–1287) Hyelim Kang, Joon-Seok Lee, Won Seok Chang and Shin-Hyun Kim Article first published online: 10 DEC 2014 | DOI: 10.1002/adma.201404706 [ABSTRACT] Omniphobic inverse opals are created by structurally and chemically modifying the surface of inverse opals through reactive ion etching. During the etching, ...
Robust Microfluidic Encapsulation of Cholesteric Liquid Crystals toward Photonic Ink Capsules,` Advanced Materials, 27, 627−633 (2015) Sang Seok Lee, Bomi Kim, Su Kyung Kim, Jong Chan Won, Yun Ho Kim, and Shin-Hyun Kim 관련기사:  http://www.hellodd.com/news/article.html?no=51837  [ABSTACT] Robust photonic microcapsules are created by microfluidic encapsulation of chol...
Cofactor-Free Light-Driven Whole-Cell Cytochrome P450 Catalysis Angew. Chem. Int. Ed. 54(3):969-973 (Jan. 12 2015)   Park JH, Lee SH, Cha GS, Choi DS, Nam DH, Lee JH, Lee JK, Yun CH, Jeong KJ*, Park, CB*  [ABSTRACT] Cytochromes P450 can catalyze various regioselective and stereospecific oxidation reactions of non-functionalized hydrocarbons. Here, we have designed a novel ...
Metabolic engineering of antibiotic factories: new tools for antibiotic production in actinomycetes Tilmann Weber, Pep Charusanti, Ewa Maria Musiol-Kroll, Xinglin Jiang, Yaojun Tong, Hyun Uk Kim, Sang Yup Lee  [ABSTRACT] Actinomycetes contain diverse antibiotic gene clusters that have not been fully explored yet. Existing and recently developed tools now allow us to perform metabolic ...
New Platform for cytochrome P450 reaction combining in situ immobilization on biopolymer.   Lee JH, Nam DH, Lee SH, Park JH, Park SJ, Lee SH, Park CB, Jeong KJ   Bioconjugate Chemistry (2014) 25(12):2101-2104.   [ABSTRACT]We describe an efficienct chemical conversion platform with in situ immobilization of P450-BM3 on poly(3-hydroxybutyrate) granules. Through fusion ...