Department of Chemical and Biomolecular Engineering
Korea Advanced Institute of Science and Technology

Recent Achievements

제목

[outside front cover] A label-free and enzyme-free signal amplification strategy for a sensitive RNase H activity assay

Title: A label-free and enzyme-free signal amplification strategy for a sensitive RNase H activity assay 

 

Author: Chang Yeol Lee, Hyowon Jang,Ki Soo Park* and Hyun Gyu Park* (: equal contribution) 

 

Journal:  Nanoscale 2017, 9, 16149-16153


Abstract: We herein describe a label-free and enzyme-free signal amplification strategy for the sensitive determination of ribonuclease H (RNase H) activity, which relies on the target-triggered catalytic hairpin assembly (CHA) in conjunction with a G-quadruplex specific fluorescent binder, N-methyl mesoporphyrin IX (NMM). In the absence of RNase H, the RNA/DNA duplex serving as a substrate for RNase H cannot initiate the execution of CHA that produces G-quadruplexes; so NMM shows a low fluorescence signal. In contrast, the presence of RNase H that degrades RNA in the RNA/DNA duplex releases DNA designed to function as the catalyst for CHA. This consequently promotes the efficient CHA and generates a large number of G-quadruplexes with a significantly enhanced fluorescence signal from NMM. Based on this label-free and enzyme-free signal amplification strategy, we successfully determined the RNase H activity with a detection limit of 0.037 U mL1 and screened potential RNase H inhibitors. Our results suggest that the developed system is a promising platform for a cost-effective, sensitive enzyme activity assay and inhibitor screening. 

 



 

 

0

추천하기

0

반대하기

첨부파일 다운로드

등록자관리자

등록일2017-11-06

조회수3,924

  • 페이스북 공유
  • 트위터 공유
  • Google+ 공유
  • 인쇄하기
 
스팸방지코드 :
사진제목
Title: Structure and function of the N-terminal domain of Ralstonia eutropha polyhydroxyalkanoate synthase, and the proposed structure and mechanisms of the whole enzyme Author: Kim, Y-J., Choi, S.Y., Kim, J.E., Jin, K.S., Lee, S.Y., Kim, K-J. Journal: Biotechnol. J., 12(1):  1-12 (2017. 1) Abstract: Polyhydroxyalkanoates (PHAs) are natural...
Title: Crystal structure of Ralstonia eutrophapolyhydroxyalkanoate synthase C-terminal domain and reaction mechanismsAuthor: Kim, J.E., Kim, Y-J., Choi, S.Y., Lee, S.Y.,Kim, K-J.Journal: Biotechnol. J., 12(1):  1-12(2017. 1)Abstract:Polyhydroxyalkanoates(PHAs) are natural polyesters synthesized by numerous microorganisms as energyand reducing power storage materials, and h...
Title: Biosynthesis of poly(2-hydroxyisovalerate-co-lactate) by metabolically engineered Escherichia coli   Author: Yang, J.E., Kim, J.W., Oh, Y.H., Choi, S.Y., Lee, H., Park, A.-R., Shin, J., Park, S.J., and Lee, S.Y.  Journal: Biotechnol. J. , 11(12): 1572-1585 (2016. 12)  Abstract: Polyhydroxyalkanoates (PHAs) containing 2-hydroxy...
Title: Fluorescent Block Copolymer-MoS2Nanocomposites for Real-Time Photothermal Heating and Imaging Author: Chan Ho Park, Hongseok Yun, Hyunseung Yang, JunhyukLee, and Bumjoon J. Kim* Journal: Advanced Functional Materials, 2017, 27, 1604403 Abstract:Novel self-monitoring photothermal (PT) agents are developed using optothermally responsive block copolymer-MoS2 ...
Title:Three-Dimensional Interlocking Interface: Mechanical Nanofastener for HighInterfacial Robustness of Polymer Electrolyte Membrane Fuel Cells Author: SeongminYuk, Min-Ju Choo, Dongyoung Lee, Hwanuk Guim, Tae-Ho Kim, Dai Gil Lee, SungyuChoi, Dong-Hyun Lee, Gisu Doo, Young Taik Hong and Hee-Tak Kim Journal:Advanced Materials, 2017, 29, 1603056 Abstract: A scalable nano-fastener fe...