Department of Chemical and Biomolecular Engineering
Korea Advanced Institute of Science and Technology

Recent Achievements


[outside front cover] A label-free and enzyme-free signal amplification strategy for a sensitive RNase H activity assay

Title: A label-free and enzyme-free signal amplification strategy for a sensitive RNase H activity assay 


Author: Chang Yeol Lee, Hyowon Jang,Ki Soo Park* and Hyun Gyu Park* (: equal contribution) 


Journal:  Nanoscale 2017, 9, 16149-16153

Abstract: We herein describe a label-free and enzyme-free signal amplification strategy for the sensitive determination of ribonuclease H (RNase H) activity, which relies on the target-triggered catalytic hairpin assembly (CHA) in conjunction with a G-quadruplex specific fluorescent binder, N-methyl mesoporphyrin IX (NMM). In the absence of RNase H, the RNA/DNA duplex serving as a substrate for RNase H cannot initiate the execution of CHA that produces G-quadruplexes; so NMM shows a low fluorescence signal. In contrast, the presence of RNase H that degrades RNA in the RNA/DNA duplex releases DNA designed to function as the catalyst for CHA. This consequently promotes the efficient CHA and generates a large number of G-quadruplexes with a significantly enhanced fluorescence signal from NMM. Based on this label-free and enzyme-free signal amplification strategy, we successfully determined the RNase H activity with a detection limit of 0.037 U mL1 and screened potential RNase H inhibitors. Our results suggest that the developed system is a promising platform for a cost-effective, sensitive enzyme activity assay and inhibitor screening. 








첨부파일 다운로드




  • 페이스북 공유
  • 트위터 공유
  • Google+ 공유
  • 인쇄하기
스팸방지코드 :
Title: Self-Organization of Polymer Additive, Poly(2-vinylpyridine) via One-Step Solution Processing to Enhance the Efficiency and Stability of Polymer Solar Cells Author: Wonho Lee, Seonju Jeong, Changyeon Lee, Gibok Han, Changsoon Cho, Jung-Yong Lee, Bumjoon J. Kim*    Journal: Advanced Energy Materials, 2017, Vol.7, 1602812 Abstract: &...
Title: A hydrocarbon/Nafion bilayer membrane with a mechanical nano-fastener for vanadium redox flow batteries   Author: Soohyun Kim, Seongmin Yuk, Hyungyu Kim, Chanyong Choi, Riyul Kim, Jang Young Lee, Young Taik Hong and Hee-Tak Kim* Journal: Journal of Materials Chemistry A, 2017, 5, 17279-17286 Inside front cover Abstra...
Title: Stimuli-Responsive, Shape-Transforming Nanostructured Particles Author: Junhyuk Lee, Kang Hee Ku, Mingoo Kim, Jae Man Shin, Junghun Han, Chan Ho Park, Gi-Ra Yi, Se Gyu Jang, Bumjoon J. Kim*   Journal: Advanced Materials, 2017, Vol.29, 1700608 Abstract Dynamic, temperature-driven transformation of the shap...
Title:  [Frontispiece] Structural Color Palettes of Core-Shell Photonic InkCapsules containing Cholesteric Liquid CrystalsAuthor: Sang Seok Lee, HyeonJin Seo, Yun Ho Kim,* and Shin-Hyun Kim*Journal: AdvancedMaterials, 2017, Vol.29, 1606894AbstractPhotonicmicrocapsules with onion-like topology are microfluidically designed to have cholesteric liquid crystals with oppositehandedness in th...
Title:  [Front cover] Selective Coloration of Melanin Nanospheres throughResonant Mie ScatteringAuthor: Soojeong Cho, Tae SoupShim, Ju Hyeon Kim, Dong-Hyun Kim, and Shin-Hyun Kim*Journal: Advanced Materials,2017, Vol.29, 1700256AbstractBlack melanin inks are preparedto selectively exhibit colors under strong light, inspired by human hair. Highabsorbance of melanin suppresses multiple sca...