Department of Chemical and Biomolecular Engineering
Korea Advanced Institute of Science and Technology

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[outside front cover] A label-free and enzyme-free signal amplification strategy for a sensitive RNase H activity assay

Title: A label-free and enzyme-free signal amplification strategy for a sensitive RNase H activity assay 

 

Author: Chang Yeol Lee, Hyowon Jang,Ki Soo Park* and Hyun Gyu Park* (: equal contribution) 

 

Journal:  Nanoscale 2017, 9, 16149-16153


Abstract: We herein describe a label-free and enzyme-free signal amplification strategy for the sensitive determination of ribonuclease H (RNase H) activity, which relies on the target-triggered catalytic hairpin assembly (CHA) in conjunction with a G-quadruplex specific fluorescent binder, N-methyl mesoporphyrin IX (NMM). In the absence of RNase H, the RNA/DNA duplex serving as a substrate for RNase H cannot initiate the execution of CHA that produces G-quadruplexes; so NMM shows a low fluorescence signal. In contrast, the presence of RNase H that degrades RNA in the RNA/DNA duplex releases DNA designed to function as the catalyst for CHA. This consequently promotes the efficient CHA and generates a large number of G-quadruplexes with a significantly enhanced fluorescence signal from NMM. Based on this label-free and enzyme-free signal amplification strategy, we successfully determined the RNase H activity with a detection limit of 0.037 U mL1 and screened potential RNase H inhibitors. Our results suggest that the developed system is a promising platform for a cost-effective, sensitive enzyme activity assay and inhibitor screening. 

 



 

 

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등록일2017-11-06

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Seungwoo Lee, Hong Suk Kang, Jung-Ki Park*Highlighted as a “Frontispiece” of Advanced Functional Materials, 21(10), 1770-1778 (2011) Article first published online: 15 MAR 2011 DOI: 10.1002/adfm.201001927 http://onlinelibrary.wiley.com/doi/10.1002/adfm.201001927/abstract Abstract A major challenge in nanolithography is to overcome the resolution limit of conventional patterni...
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