Department of Chemical and Biomolecular Engineering
Korea Advanced Institute of Science and Technology

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[cover paper] Polyketide bio-derivatization using the promiscuous acyltransferase KirCII

Title: Polyketide bio-derivatization using the promiscuous acyltransferase KirCII


Author: Musiol-Kroll, E.M., Zubeil, F., Schafhauser, T., Hartner, T., Kulik, A., McArthur, J., Koryakina, I., Wohlleben, W., Grond, S., Williams, G.J., Lee, S.Y., and Weber, T.


Journal: ACS Synth. Biol., 2017, 6 (3), pp 421427 

 

Abstract:

During polyketide biosynthesis, acyltransferases (ATs) are the essential gatekeepers which provide the assembly lines with precursors and thus contribute greatly to structural diversity. Previously, we demonstrated that the discrete AT KirCII from the kirromycin antibiotic pathway accesses nonmalonate extender units. Here, we exploit the promiscuity of KirCII to generate new kirromycins with allyl- and propargyl-side chains in vivo, the latter were utilized as educts for further modification by click chemistry. 

 

 

 

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