Department of Chemical and Biomolecular Engineering
Korea Advanced Institute of Science and Technology

Recent Achievements


[cover paper] Structure and function of the N-terminal domain of Ralstonia eutropha polyhydroxyalkanoate synthase, and the proposed structure and mechanisms of the whole enzyme

Title: Structure and function of the N-terminal domain of Ralstonia eutropha polyhydroxyalkanoate synthase, and the proposed structure and mechanisms of the whole enzyme

Author: Kim, Y-J., Choi, S.Y., Kim, J.E., Jin, K.S., Lee, S.Y., Kim, K-J.

Journal: Biotechnol. J., 12(1):  1-12 (2017. 1)


Polyhydroxyalkanoates (PHAs) are natural polyesters synthesized by numerous microorganisms as energy and reducing power storage materials, and have attracted much attention as substitutes for petroleum-based plastics. In an accompanying paper, the authors reported the crystal structure of the C-terminal domain of Ralstonia eutropha PHA synthase (PhaC1). Here, the authors report the 3D reconstructed model of full-length of R. eutropha PhaC1 (RePhaC1F) by small angle X-ray scattering (SAXS) analysis. The catalytic C-terminal domain of RePhaC1 (RePhaC1CD) dimer is located at the center of RePhaC1F, and the N-terminal domain of RePhaC1 (RePhaC1ND) is located opposite the dimerization subdomain of RePhaC1CD, indicating that RePhaC1ND is not directly involved in the enzyme catalysis. The localization studies using RePhaC1F, RePhaC1ND and RePhaC1CD revealed that RePhaC1ND plays important roles in PHA polymerization by localizing the enzyme to the PHA granules and stabilizing the growing PHA polymer near the active site of RePhaC1CD. The serial truncation study on RePhaC1ND suggested that the predicted five α-helices (N-α3 to N-α7) are required for proper folding and granule binding function of RePhaC1ND. In addition, the authors also report the SAXS 3D reconstructed model of the RePhaC1F/RePhaMΔC complex (RePhaMΔC, PAKKA motif-truncated version of RePhaM). RePhaM forms a complex with RePhaC1 by interacting with RePhaC1ND and activates RePhaC1 by providing a more extensive surface area for interaction with the growing PHA polymer.






첨부파일 다운로드




  • 페이스북 공유
  • 트위터 공유
  • Google+ 공유
  • 인쇄하기
스팸방지코드 :
Microcapsules: Osmocapsules for Direct Measurement of Osmotic Strength (Small 6/2014) (page 1232) Shin-Hyun Kim, Tae Yong Lee and Sang Seok Lee Article first published online: 24 MAR 2014 | DOI: 10.1002/smll.201470037 [ABSTRACT] Osmocapsules are microfluidically created to provide a tool for direct measurement of osmotic pressure. As reported by S.-H. Kim and co-workers on page 1155,...
Hierarchical Structures: 3D Hierarchical Architectures Prepared by Single Exposure Through a Highly Durable Colloidal Phase Mask (Adv. Mater. 9/2014) Tae Yoon Jeon, Hwan Chul Jeon, Su Yeon Lee, Tae Soup Shim, Jung-Dae Kwon, Sung-Gyu Park, Seung-Man Yang Article first published online: 4 MAR 2014 DOI: 10.1002/adma.201470058 [ABSTRACT] A simple and cost-effective fabrication of 3...
Droplet Microfluidics for Producing Functional Microparticles  Ju Hyeon Kim, Tae Yoon Jeon, Tae Min Choi, Tae Soup Shim, Shin-Hyun Kim, and Seung-Man Yang [ABSTRACT] Isotropic microparticles prepared from a suspension that undergoes polymerization have long been used for a variety ofapplications. Bulk emulsification procedures produce polydisperse emulsion droplets that are transfor...
Tuning Cage Dimension in Clathrate Hydrates for Hydrogen Multiple Occupancy Dong-Yeun Koh †, Hyery Kang †, Jiwon Jeon ‡, Yun-Ho Ahn †, Youngjune Park †, Hyungjun Kim *‡, and Huen Lee *†‡ †Department of Chemical and Biomolecular Enginieering and ‡Graduate School of EEWS, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daej...
Microbial production of short-chain alkanes Yong Jun Choi & Sang Yup Lee Nature Volume:502,Pages:571–574Date published:(24 October 2013)DOI:doi:10.1038/nature12536 Increasing concerns about limited fossil fuels and global environmental problems have focused attention on the need to develop sustainable biofuels from renewable resources. Although microbial production of diesel...