Department of Chemical and Biomolecular Engineering
Korea Advanced Institute of Science and Technology

Recent Achievements

제목

[cover paper] Structure and function of the N-terminal domain of Ralstonia eutropha polyhydroxyalkanoate synthase, and the proposed structure and mechanisms of the whole enzyme

Title: Structure and function of the N-terminal domain of Ralstonia eutropha polyhydroxyalkanoate synthase, and the proposed structure and mechanisms of the whole enzyme


Author: Kim, Y-J., Choi, S.Y., Kim, J.E., Jin, K.S., Lee, S.Y., Kim, K-J.


Journal: Biotechnol. J., 12(1):  1-12 (2017. 1)


Abstract:

Polyhydroxyalkanoates (PHAs) are natural polyesters synthesized by numerous microorganisms as energy and reducing power storage materials, and have attracted much attention as substitutes for petroleum-based plastics. In an accompanying paper, the authors reported the crystal structure of the C-terminal domain of Ralstonia eutropha PHA synthase (PhaC1). Here, the authors report the 3D reconstructed model of full-length of R. eutropha PhaC1 (RePhaC1F) by small angle X-ray scattering (SAXS) analysis. The catalytic C-terminal domain of RePhaC1 (RePhaC1CD) dimer is located at the center of RePhaC1F, and the N-terminal domain of RePhaC1 (RePhaC1ND) is located opposite the dimerization subdomain of RePhaC1CD, indicating that RePhaC1ND is not directly involved in the enzyme catalysis. The localization studies using RePhaC1F, RePhaC1ND and RePhaC1CD revealed that RePhaC1ND plays important roles in PHA polymerization by localizing the enzyme to the PHA granules and stabilizing the growing PHA polymer near the active site of RePhaC1CD. The serial truncation study on RePhaC1ND suggested that the predicted five α-helices (N-α3 to N-α7) are required for proper folding and granule binding function of RePhaC1ND. In addition, the authors also report the SAXS 3D reconstructed model of the RePhaC1F/RePhaMΔC complex (RePhaMΔC, PAKKA motif-truncated version of RePhaM). RePhaM forms a complex with RePhaC1 by interacting with RePhaC1ND and activates RePhaC1 by providing a more extensive surface area for interaction with the growing PHA polymer.


 

0

추천하기

0

반대하기

첨부파일 다운로드

등록자관리자

등록일2017-04-11

조회수944

  • 페이스북 공유
  • 트위터 공유
  • Google+ 공유
  • 인쇄하기
 
스팸방지코드 :
사진제목
Seungwoo Lee, Jonghwa Shin, Hong Suk Kang, Yong-Hee Lee, Jung-Ki Park* Highlighted as a “Frontispiece” of Advanced Materials, 23(29), 3244-3250 (2011) Article first published online: 3 JUN 2011 DOI: 10.1002/adma.201100662  http://onlinelibrary.wiley.com/doi/10.1002/adma.201100662/abstract Abstract Directional photofluidization lithography enables deterministic large-area na...
Seungwoo Lee, Hong Suk Kang, Jung-Ki Park*Highlighted as a “Frontispiece” of Advanced Functional Materials, 21(10), 1770-1778 (2011) Article first published online: 15 MAR 2011 DOI: 10.1002/adfm.201001927 http://onlinelibrary.wiley.com/doi/10.1002/adfm.201001927/abstract Abstract A major challenge in nanolithography is to overcome the resolution limit of conventional patterni...
Shin-Hyun Kim, Hyerim Hwang, Che Ho Lim, Jae Won Shim, Seung-Man Yang Article first published online: 1 MAR 2011 DOI: 10.1002/adfm.201002316 http://onlinelibrary.wiley.com/doi/10.1002/adfm.201002316/abstract Abstract Advances in microfluidic emulsification have enabled the creation of multiphase emulsion drops, which have emerged as promising templates for producing functional micro...
Byoung Yeon Won, Sujeong Shin, Songyi Baek, Ye Lim Jung, Taihua Li, Sung Chul Shin, Dae-Yeon Cho, Sang Bok Lee and Hyun Gyu ParkAnalyst, 2011, 136, 1573-1579DOI: 10.1039/C0AN00695E Received 07 Sep 2010, Accepted 21 Jan 2011First published on the web 14 Feb 2011http://pubs.rsc.org/en/Content/ArticleLanding/2011/AN/c0an00695e Abstract The operation of an electrochemical real-time PCR syste...
Min-Ah Woo†, Moon Il Kim†, Byung Jo Yu‡, Daeyeon Cho§, Nag-Jong Kim†, June Hyoung Cho‡, Byung-Ok Choi*, Ho Nam Chang†, and Hyun Gyu Park*† † Department of Chemical and Biomolecular Engineering (BK21 Program), KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea‡ MD Science Inc., 258-1 Munji-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea http://pubs...