Department of Chemical and Biomolecular Engineering
Korea Advanced Institute of Science and Technology

Recent Achievements

제목

[cover paper] Structure and function of the N-terminal domain of Ralstonia eutropha polyhydroxyalkanoate synthase, and the proposed structure and mechanisms of the whole enzyme

Title: Structure and function of the N-terminal domain of Ralstonia eutropha polyhydroxyalkanoate synthase, and the proposed structure and mechanisms of the whole enzyme


Author: Kim, Y-J., Choi, S.Y., Kim, J.E., Jin, K.S., Lee, S.Y., Kim, K-J.


Journal: Biotechnol. J., 12(1):  1-12 (2017. 1)


Abstract:

Polyhydroxyalkanoates (PHAs) are natural polyesters synthesized by numerous microorganisms as energy and reducing power storage materials, and have attracted much attention as substitutes for petroleum-based plastics. In an accompanying paper, the authors reported the crystal structure of the C-terminal domain of Ralstonia eutropha PHA synthase (PhaC1). Here, the authors report the 3D reconstructed model of full-length of R. eutropha PhaC1 (RePhaC1F) by small angle X-ray scattering (SAXS) analysis. The catalytic C-terminal domain of RePhaC1 (RePhaC1CD) dimer is located at the center of RePhaC1F, and the N-terminal domain of RePhaC1 (RePhaC1ND) is located opposite the dimerization subdomain of RePhaC1CD, indicating that RePhaC1ND is not directly involved in the enzyme catalysis. The localization studies using RePhaC1F, RePhaC1ND and RePhaC1CD revealed that RePhaC1ND plays important roles in PHA polymerization by localizing the enzyme to the PHA granules and stabilizing the growing PHA polymer near the active site of RePhaC1CD. The serial truncation study on RePhaC1ND suggested that the predicted five α-helices (N-α3 to N-α7) are required for proper folding and granule binding function of RePhaC1ND. In addition, the authors also report the SAXS 3D reconstructed model of the RePhaC1F/RePhaMΔC complex (RePhaMΔC, PAKKA motif-truncated version of RePhaM). RePhaM forms a complex with RePhaC1 by interacting with RePhaC1ND and activates RePhaC1 by providing a more extensive surface area for interaction with the growing PHA polymer.


 

0

추천하기

0

반대하기

첨부파일 다운로드

등록자관리자

등록일2017-04-11

조회수950

  • 페이스북 공유
  • 트위터 공유
  • Google+ 공유
  • 인쇄하기
 
스팸방지코드 :
사진제목
Simple and Universal Platform for Logic Gate Operations Based on Molecular Beacon Probes (Small 14/2012 Ki Soo Park, Myung Wan Seo, Cheulhee Jung, Joon Young Lee, Hyun Gyu Park Article first published online: 13 JUL 2012 DOI: 10.1002/smll.201290077 The cover picture illustrates how the hairpin-structured molecular beacon probe interacts with DNA input and generates a high ...
  Nanoscale, 2012, 4, 3879–3885 DOI: 10.1039/C2NR30168G First published on the web 03 Apr 2012   Gle Park,a Leian Bartolome,b Kyoung G. Lee,a Seok Jae Lee,a Do Hyun Kim*b and Tae Jung Park*a    a: Center for Nanobio Integration & Convergence Engineering (NICE), National Nanofab Center    b: Department of Chemical & Biomolecula...
  Advanced Energy MaterialsVolume 2, Issue 6, pages 645–650, June, 2012  Myung-Hyun Ryou, Dong Jin Lee,Je-Nam Lee, Yong Min Lee, Jung-Ki Park, Jang Wook Choi, Article first published online: 13 APR 2012 DOI: 10.1002/aenm.201100687 An excellent cycle life (150 cycles with 80% retention) for lithium-metal anodes in lithium-ion batteries is achieved by empl...
A microfluidic droplet generator uniformly encapsulates an equivalent number ofengineered E. coli cells and the same Fe and Mn metal ion concentrations inidentically sized droplets. In their Communication (DOI: 10.1002/anie.201208977),T. S. Seo, S. Y. Lee, and co-workers demonstrate the synthesis of homogeneousbiogenic paramagnetic nanoparticles inside recombinant E. coli cells by encapsul...
Mismatched pyrrolo-dC-modified duplex DNA as a novel probe for sensitive detection of silver ions Ki Soo Park ,  Joon Young Lee and Hyun Gyu Park  Chem. Commun., 2012, 48, 4549-4551 DOI: 10.1039/C2CC17148A Abstract A new technology that enables the highly selective and sensitive detection of silver ions has been developed. The method takes advantage of the unique fluorescence...