Department of Chemical and Biomolecular Engineering
Korea Advanced Institute of Science and Technology

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[cover paper] Crystal structure of Ralstonia eutropha polyhydroxyalkanoate synthase C-terminal domain and reaction mechanisms

Title: Crystal structure of Ralstonia eutrophapolyhydroxyalkanoate synthase C-terminal domain and reaction mechanisms


Author: Kim, J.E., Kim, Y-J., Choi, S.Y., Lee, S.Y.,Kim, K-J.


Journal: Biotechnol. J., 12(1):  1-12(2017. 1)


Abstract:

Polyhydroxyalkanoates(PHAs) are natural polyesters synthesized by numerous microorganisms as energyand reducing power storage materials, and have attracted much attention assubstitutes for petroleum-based plastics. Here, we report the first crystalstructure of Ralstonia eutropha PHA synthase at 1.8 Å resolution and structure-based mechanisms for PHApolymerization. RePhaC1 contains two distinct domains, the N-terminal (RePhaC1ND)and C-terminal domains (RePhaC1CD), and exists as a dimer. RePhaC1CDcatalyzes polymerization via non-processive ping-pong mechanism using aCys-His-Asp catalytic triad. Molecular docking simulation of3-hydroxybutyryl-CoA to the active site of RePhaC1CD revealsresidues involved in the formation of 3-hydroxybutyryl-CoA binding pocket andsubstrate binding tunnel. Comparative analysis with other polymeraseselucidates how different classes of PHA synthases show different substratespecificities. Furthermore, we attempted structure-based protein engineeringand developed a RePhaC1 mutant with enhanced PHA synthase activity.


 



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등록일2017-04-11

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Title: Structure and function of the N-terminal domain of Ralstonia eutropha polyhydroxyalkanoate synthase, and the proposed structure and mechanisms of the whole enzyme Author: Kim, Y-J., Choi, S.Y., Kim, J.E., Jin, K.S., Lee, S.Y., Kim, K-J. Journal: Biotechnol. J., 12(1):  1-12 (2017. 1) Abstract: Polyhydroxyalkanoates (PHAs) are natural...
Title: Crystal structure of Ralstonia eutrophapolyhydroxyalkanoate synthase C-terminal domain and reaction mechanismsAuthor: Kim, J.E., Kim, Y-J., Choi, S.Y., Lee, S.Y.,Kim, K-J.Journal: Biotechnol. J., 12(1):  1-12(2017. 1)Abstract:Polyhydroxyalkanoates(PHAs) are natural polyesters synthesized by numerous microorganisms as energyand reducing power storage materials, and h...
Title: Biosynthesis of poly(2-hydroxyisovalerate-co-lactate) by metabolically engineered Escherichia coli   Author: Yang, J.E., Kim, J.W., Oh, Y.H., Choi, S.Y., Lee, H., Park, A.-R., Shin, J., Park, S.J., and Lee, S.Y.  Journal: Biotechnol. J. , 11(12): 1572-1585 (2016. 12)  Abstract: Polyhydroxyalkanoates (PHAs) containing 2-hydroxy...
Title: Fluorescent Block Copolymer-MoS2Nanocomposites for Real-Time Photothermal Heating and Imaging Author: Chan Ho Park, Hongseok Yun, Hyunseung Yang, JunhyukLee, and Bumjoon J. Kim* Journal: Advanced Functional Materials, 2017, 27, 1604403 Abstract:Novel self-monitoring photothermal (PT) agents are developed using optothermally responsive block copolymer-MoS2 ...
Title:Three-Dimensional Interlocking Interface: Mechanical Nanofastener for HighInterfacial Robustness of Polymer Electrolyte Membrane Fuel Cells Author: SeongminYuk, Min-Ju Choo, Dongyoung Lee, Hwanuk Guim, Tae-Ho Kim, Dai Gil Lee, SungyuChoi, Dong-Hyun Lee, Gisu Doo, Young Taik Hong and Hee-Tak Kim Journal:Advanced Materials, 2017, 29, 1603056 Abstract: A scalable nano-fastener fe...