Department of Chemical and Biomolecular Engineering
Korea Advanced Institute of Science and Technology

Recent Achievements

제목

[cover paper] Crystal structure of Ralstonia eutropha polyhydroxyalkanoate synthase C-terminal domain and reaction mechanisms

Title: Crystal structure of Ralstonia eutrophapolyhydroxyalkanoate synthase C-terminal domain and reaction mechanisms


Author: Kim, J.E., Kim, Y-J., Choi, S.Y., Lee, S.Y.,Kim, K-J.


Journal: Biotechnol. J., 12(1):  1-12(2017. 1)


Abstract:

Polyhydroxyalkanoates(PHAs) are natural polyesters synthesized by numerous microorganisms as energyand reducing power storage materials, and have attracted much attention assubstitutes for petroleum-based plastics. Here, we report the first crystalstructure of Ralstonia eutropha PHA synthase at 1.8 Å resolution and structure-based mechanisms for PHApolymerization. RePhaC1 contains two distinct domains, the N-terminal (RePhaC1ND)and C-terminal domains (RePhaC1CD), and exists as a dimer. RePhaC1CDcatalyzes polymerization via non-processive ping-pong mechanism using aCys-His-Asp catalytic triad. Molecular docking simulation of3-hydroxybutyryl-CoA to the active site of RePhaC1CD revealsresidues involved in the formation of 3-hydroxybutyryl-CoA binding pocket andsubstrate binding tunnel. Comparative analysis with other polymeraseselucidates how different classes of PHA synthases show different substratespecificities. Furthermore, we attempted structure-based protein engineeringand developed a RePhaC1 mutant with enhanced PHA synthase activity.


 



0

추천하기

0

반대하기

첨부파일 다운로드

등록자관리자

등록일2017-04-11

조회수559

  • 페이스북 공유
  • 트위터 공유
  • Google+ 공유
  • 인쇄하기
 
스팸방지코드 :
사진제목
Byoung Yeon Won, Sujeong Shin, Songyi Baek, Ye Lim Jung, Taihua Li, Sung Chul Shin, Dae-Yeon Cho, Sang Bok Lee and Hyun Gyu ParkAnalyst, 2011, 136, 1573-1579DOI: 10.1039/C0AN00695E Received 07 Sep 2010, Accepted 21 Jan 2011First published on the web 14 Feb 2011http://pubs.rsc.org/en/Content/ArticleLanding/2011/AN/c0an00695e Abstract The operation of an electrochemical real-time PCR syste...
Min-Ah Woo†, Moon Il Kim†, Byung Jo Yu‡, Daeyeon Cho§, Nag-Jong Kim†, June Hyoung Cho‡, Byung-Ok Choi*, Ho Nam Chang†, and Hyun Gyu Park*† † Department of Chemical and Biomolecular Engineering (BK21 Program), KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea‡ MD Science Inc., 258-1 Munji-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea http://pubs...
Ki Soo Park, Cheulhee Jung and Hyun Gyu Park Angewandte Chemie International Edition, 49(50), 9757-9760, (2010.12.10) Article first published online: 26 OCT 2010 DOI: 10.1002/anie.201004406 http://onlinelibrary.wiley.com/doi/10.1002/anie.201004406/abstract Abstract Metal ion, an astonishing illusionist: In a new concept, “illusionary” polymerase activity is intentionally...