Department of Chemical and Biomolecular Engineering
Korea Advanced Institute of Science and Technology

Recent Achievements

제목

[cover paper] Crystal structure of Ralstonia eutropha polyhydroxyalkanoate synthase C-terminal domain and reaction mechanisms

Title: Crystal structure of Ralstonia eutrophapolyhydroxyalkanoate synthase C-terminal domain and reaction mechanisms


Author: Kim, J.E., Kim, Y-J., Choi, S.Y., Lee, S.Y.,Kim, K-J.


Journal: Biotechnol. J., 12(1):  1-12(2017. 1)


Abstract:

Polyhydroxyalkanoates(PHAs) are natural polyesters synthesized by numerous microorganisms as energyand reducing power storage materials, and have attracted much attention assubstitutes for petroleum-based plastics. Here, we report the first crystalstructure of Ralstonia eutropha PHA synthase at 1.8 Å resolution and structure-based mechanisms for PHApolymerization. RePhaC1 contains two distinct domains, the N-terminal (RePhaC1ND)and C-terminal domains (RePhaC1CD), and exists as a dimer. RePhaC1CDcatalyzes polymerization via non-processive ping-pong mechanism using aCys-His-Asp catalytic triad. Molecular docking simulation of3-hydroxybutyryl-CoA to the active site of RePhaC1CD revealsresidues involved in the formation of 3-hydroxybutyryl-CoA binding pocket andsubstrate binding tunnel. Comparative analysis with other polymeraseselucidates how different classes of PHA synthases show different substratespecificities. Furthermore, we attempted structure-based protein engineeringand developed a RePhaC1 mutant with enhanced PHA synthase activity.


 



0

추천하기

0

반대하기

첨부파일 다운로드

등록자관리자

등록일2017-04-11

조회수552

  • 페이스북 공유
  • 트위터 공유
  • Google+ 공유
  • 인쇄하기
 
스팸방지코드 :
사진제목
A microfluidic droplet generator uniformly encapsulates an equivalent number ofengineered E. coli cells and the same Fe and Mn metal ion concentrations inidentically sized droplets. In their Communication (DOI: 10.1002/anie.201208977),T. S. Seo, S. Y. Lee, and co-workers demonstrate the synthesis of homogeneousbiogenic paramagnetic nanoparticles inside recombinant E. coli cells by encapsul...
Mismatched pyrrolo-dC-modified duplex DNA as a novel probe for sensitive detection of silver ions Ki Soo Park ,  Joon Young Lee and Hyun Gyu Park  Chem. Commun., 2012, 48, 4549-4551 DOI: 10.1039/C2CC17148A Abstract A new technology that enables the highly selective and sensitive detection of silver ions has been developed. The method takes advantage of the unique fluorescence...
Controlled generation of submicron emulsion droplets via highly stable tip-streaming mode in microfluidic devices Woong-Chan Jeong ,  Jong-Min Lim ,  Jae-Hoon Choi ,  Jong-Hoon Kim ,  You-Jin Lee ,  Seung-Hyun Kim ,  Gaehang Lee ,  Jong-Duk Kim ,  Gi-Ra Yi and Seung-Man Yang Lab Chip, 2012, 12, 1446-1453DOI: 10.1039/C2LC00018K ABSTRACT S...
  Hong Suk Kang,* Seungwoo Lee,* and Jung-Ki Park, `Monolithic, hierarchical surface reliefs by holographic photofluidization of azopolymer arrays: Direct visualization of polymeric flows,` Advanced Functional Materials, 2011, 21, 4412 (*denote equal contribution)   ABSTRACT The structural features of monolithically ordered, hierarchical surface reliefs can be determ...
Tae Soup Shim, Dr. Shin-Hyun Kim, Dr. Chul-Joon Heo, Hwan Chul Jeon and Prof. Seung-Man Yang Article first published online: 8 DEC 2011 | DOI: 10.1002/anie.201108216 Abstract Origami of hydrogel bilayers provides robust mircocapsules through anisotropic volume expansion. In their Communication on page 1420 ff., S.-M. Yang and co-workers show planar bilayer microparticles composed...
첫 페이지로 이동 10 페이지로 이동 이전 페이지로 이동   11    12    13    14   다음 페이지로 이동 마지막 페이지로 이동