Department of Chemical and Biomolecular Engineering
Korea Advanced Institute of Science and Technology

Recent Achievements


[cover paper] Crystal structure of Ralstonia eutropha polyhydroxyalkanoate synthase C-terminal domain and reaction mechanisms

Title: Crystal structure of Ralstonia eutrophapolyhydroxyalkanoate synthase C-terminal domain and reaction mechanisms

Author: Kim, J.E., Kim, Y-J., Choi, S.Y., Lee, S.Y.,Kim, K-J.

Journal: Biotechnol. J., 12(1):  1-12(2017. 1)


Polyhydroxyalkanoates(PHAs) are natural polyesters synthesized by numerous microorganisms as energyand reducing power storage materials, and have attracted much attention assubstitutes for petroleum-based plastics. Here, we report the first crystalstructure of Ralstonia eutropha PHA synthase at 1.8 Å resolution and structure-based mechanisms for PHApolymerization. RePhaC1 contains two distinct domains, the N-terminal (RePhaC1ND)and C-terminal domains (RePhaC1CD), and exists as a dimer. RePhaC1CDcatalyzes polymerization via non-processive ping-pong mechanism using aCys-His-Asp catalytic triad. Molecular docking simulation of3-hydroxybutyryl-CoA to the active site of RePhaC1CD revealsresidues involved in the formation of 3-hydroxybutyryl-CoA binding pocket andsubstrate binding tunnel. Comparative analysis with other polymeraseselucidates how different classes of PHA synthases show different substratespecificities. Furthermore, we attempted structure-based protein engineeringand developed a RePhaC1 mutant with enhanced PHA synthase activity.






첨부파일 다운로드




  • 페이스북 공유
  • 트위터 공유
  • Google+ 공유
  • 인쇄하기
스팸방지코드 :
Tuning Cage Dimension in Clathrate Hydrates for Hydrogen Multiple Occupancy Dong-Yeun Koh †, Hyery Kang †, Jiwon Jeon ‡, Yun-Ho Ahn †, Youngjune Park †, Hyungjun Kim *‡, and Huen Lee *†‡ †Department of Chemical and Biomolecular Enginieering and ‡Graduate School of EEWS, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daej...
Microbial production of short-chain alkanes Yong Jun Choi & Sang Yup Lee Nature Volume:502,Pages:571–574Date published:(24 October 2013)DOI:doi:10.1038/nature12536 Increasing concerns about limited fossil fuels and global environmental problems have focused attention on the need to develop sustainable biofuels from renewable resources. Although microbial production of diesel...
Title: Synthesis of a 3D graphite microball using a microfluidic droplet generator and its polymer composite with core–shell structure Author: Dong Ju Han, Jae Hwan Jung, Jong Seob Choi, Yong Tae Kim and Tae Seok Seo Journal : Lab on a Chip 2013,13, 4006 Lab Chip, 2013,13, 4006-4010 DOI: 10.1039/C3LC50838B ABSTRACT Spherical 3D graphite microballs (3D GMs) and their nanohybri...
Design and use of synthetic regulatory small RNAs to control gene expression in Escherichia coli Seung Min Yoo,1, 2 Dokyun Na1, 2, 3 & Sang Yup Lee1 Journal name:Nature Protocols Volume:8, Pages: 1694–1707 Year published:(2013)DOI: doi:10.1038/nprot.2013.105 Published online 08 August 2013 Abstract Gene knockout experiments are often essential in functional genomics and metabolic...
Abstract: Pristine graphene quantum dots and graphene oxide quantum dots are synthesized by chemical exfoliation from graphite nanoparticles with high uniformity in terms of shape (circle), size (less than 4 nm), and thickness (monolayer). The origin of the blue and green photoluminescence of GQDs and GOQDs is attributed to intrinsic and extrinsic energy states, respectively. Further d...