Department of Chemical and Biomolecular Engineering
Korea Advanced Institute of Science and Technology

Recent Achievements

제목

[Cover parper] Metabolic Engineering of Escherichia coli for the Production of 3‑Hydroxypropionic Acid and Malonic Acid through β‑Alanine Route

Title: Metabolic Engineering of Escherichia coli for the Production of

3Hydroxypropionic Acid and Malonic Acid through βAlanine Route

 

Author: Chan Woo Song, Je Woong Kim, In Jin Cho, and Sang Yup Lee

 

Journal: ACS Syn. Biol., 5(11): 1256-1263 (2016. 11)

 

ABSTRACT: Escherichia coli was metabolically engineered to produce industrially important platform chemicals, 3-hydroxypropionic acid (3-HP) and malonic acid (MA), through the β-alanine (BA) route. First, various combinations of downstream enzymes were screened and BA pyruvate transaminase (encoded by pa0132) from P. aeruginosa was selected to generate malonic semialdehyde (MSA) from BA.  

This platform strain was engineered by introducing E. coli MSA reductase (encoded by ydfG) to reduce MSA to 3-HP. Replacement of native promoter of the sdhC gene with the strong trc promoter in the genome increased 3-HP production to 3.69 g/L in flask culture. Introduction of E. coli semialdehyde dehydrogenase (encoded by yneI) into the platform strain resulted in the production of MA, and additional deletion of the ydfG gene increased MA production to 0.450 g/L in flask culture. Fed-batch cultures of final engineered strains resulted in the production of 31.1 g/L 3-HP or 3.60 g/L MA from glucose. 

 

       

0

추천하기

0

반대하기

첨부파일 다운로드

등록자관리자

등록일2016-12-06

조회수997

  • 페이스북 공유
  • 트위터 공유
  • Google+ 공유
  • 인쇄하기
 
스팸방지코드 :
사진제목
Title: Fluorescent Block Copolymer-MoS2Nanocomposites for Real-Time Photothermal Heating and Imaging Author: Chan Ho Park, Hongseok Yun, Hyunseung Yang, JunhyukLee, and Bumjoon J. Kim* Journal: Advanced Functional Materials, 2017, 27, 1604403 Abstract:Novel self-monitoring photothermal (PT) agents are developed using optothermally responsive block copolymer-MoS2 ...
Title:Three-Dimensional Interlocking Interface: Mechanical Nanofastener for HighInterfacial Robustness of Polymer Electrolyte Membrane Fuel Cells Author: SeongminYuk, Min-Ju Choo, Dongyoung Lee, Hwanuk Guim, Tae-Ho Kim, Dai Gil Lee, SungyuChoi, Dong-Hyun Lee, Gisu Doo, Young Taik Hong and Hee-Tak Kim Journal:Advanced Materials, 2017, 29, 1603056 Abstract: A scalable nano-fastener fe...
Title: Metabolic Engineering of Escherichia coli for the Production of 3‑Hydroxypropionic Acid and Malonic Acid through β‑Alanine Route   Author: Chan Woo Song, Je Woong Kim, In Jin Cho, and Sang Yup Lee   Journal: ACS Syn. Biol., 5(11): 1256-1263 (2016. 11)   ABSTRACT: Escherichia coli was metabolically engineered t...
Title: A Low-Voltage Organic Complementary Inverter with High Operation Stability and Flexibility Using an Ultrathin iCVD Polymer Dielectric and a Hybrid Encapsulation LayerAuthor: Hyejeong Seong, Junhwan Choi, Byung Chul Jang, Mincheol Kim, Seunghyup Yoo, Sung-Yool Choi, and Sung Gap Im* Journal: Advanced Electronic Materials, 2016, Vol.2, p.1500385  Abstract: A low-volta...
Title: Synthesis of Ultrathin,Homogeneous Copolymer Dielectrics to Control the Threshold Voltage of OrganicThin-Film Transistors Author: Kwanyong Pak , Hyejeong Seong , Junhwan Choi , Wan Sik Hwang , and Sung Gap Im*Journal: Advanced Functional materials, 2016, Vol.26, p.6574–6582AbstractThis work demonstrates that thresholdvoltage (VT) of organicthin-fi lm transistors (OTFTs) can be contro...