Department of Chemical and Biomolecular Engineering
Korea Advanced Institute of Science and Technology

Recent Achievements

제목

[Back cover] Cofactor-Free Light-Driven Whole-Cell Cytochrome P450 Catalysis

Cofactor-Free Light-Driven Whole-Cell Cytochrome P450 Catalysis

Angew. Chem. Int. Ed. 54(3):969-973 (Jan. 12 2015)  

Park JH, Lee SH, Cha GS, Choi DS, Nam DH, Lee JH, Lee JK, Yun CH, Jeong KJ*, Park, CB*

 

[ABSTRACT]

Cytochromes P450 can catalyze various regioselective and stereospecific oxidation reactions of non-functionalized hydrocarbons. Here, we have designed a novel light-driven platform for cofactor-free, whole-cell P450 photo-biocatalysis using eosinY (EY) as a photosensitizer. EY can easily enter into the cytoplasm of Escherichia coli and bind specifically to the heme domain of P450. The catalytic turnover of P450 was mediated through the direct transfer of photoinduced electrons from the photosensitized EY to the P450 heme domain under visible light illumination. The photoactivation of the P450 catalytic cycle in the absence of cofactors and redox partners is successfully conducted using many bacterial P450s (variants of P450 BM3) and human P450s (CYPs 1A1, 1A2, 1B1, 2A6, 2E1, and 3A4) for the bioconversion of different substrates, including marketed drugs (simvastatin, lovastatin, and omeprazole) and a steroid (17β-estradiol), to demonstrate the general applicability of the light-driven, cofactor-free system.

0

추천하기

0

반대하기

첨부파일 다운로드

등록자배성순

등록일2015-01-15

조회수5,559

  • 페이스북 공유
  • 트위터 공유
  • Google+ 공유
  • 인쇄하기
 
스팸방지코드 :
사진제목
Title: Metal ion triggers for reversible switching of DNA polymeraseAuthors: Ki Soo Park, Chang Yeol Lee, and Hyun Gyu Park Journal: Chemical CommunicationsPage: Vol. 52, page 4868-4871 (April, 2016) [Abstract] We herein develop a new strategy that modulates DNA polymerase activity in a reversible and switchable manner by using the novel interactions between DNA bases and metal...
Tuning selectivity of electrochemical reactions by atoomically dispersed platinum catalyst Chang Hyuck Choi, Minho Kim, Han Chang Kwon, Sung June Cho, Seongho Yun, Hee-Tak Kim, Karl J.J. Mayrhofer, Hyungjun Kim, Minkee Choi Nature Communications, Vol. 7, page 10922 (March 8, 2016) [Abstract] Maximum atom efficiency as well as distinct chemoselectivity is expected for electrocat...
Sustainable Redox Mediation for Lithium–Oxygen Batteries by a Composite Protective Layer on the Lithium-Metal Anode   Dong Jin Lee†, Hongkyung Lee†, Yun-Jung Kim , Jung-Ki Park ,* and Hee-Tak Kim* († denotes equal contribution)   Advanced Materials, Vol. 28, Issue 5, page 857-863 (February 3, 2016) [Abstract]A synergic combination of a soluble &sh...
Liquid-Impermeable Inverse Opals with Invariant Photonic Bandgap (pages 1282–1287) Hyelim Kang, Joon-Seok Lee, Won Seok Chang and Shin-Hyun Kim Article first published online: 10 DEC 2014 | DOI: 10.1002/adma.201404706 [ABSTRACT] Omniphobic inverse opals are created by structurally and chemically modifying the surface of inverse opals through reactive ion etching. During the etching, ...
Robust Microfluidic Encapsulation of Cholesteric Liquid Crystals toward Photonic Ink Capsules,` Advanced Materials, 27, 627−633 (2015) Sang Seok Lee, Bomi Kim, Su Kyung Kim, Jong Chan Won, Yun Ho Kim, and Shin-Hyun Kim 관련기사:  http://www.hellodd.com/news/article.html?no=51837  [ABSTACT] Robust photonic microcapsules are created by microfluidic encapsulation of chol...