Hyun Gyu Park (박현규)Professor
Tel : +82-42-350-3932
Fax : +82-42-350-3910
E-mail : firstname.lastname@example.org
Homepage : http://bcbd.kaist.ac.kr
- 1996 : KAIST (Ph.D. in Chem. Eng.)
- 1992 : KAIST (M.S. in Chem. Eng.)
- 1990 : KAIST (B.S. in Chem. Eng.)
Employment and Professional Experience
- August 1992 - Feb. 1993 : Visiting Scholar at Dept. of Biochem. Eng. Univ. of Iowa, USA.
- Feb. 1, 1996 – Mar. 19, 2002 : Senior Researcher at Samsung Advanced Institute of Technology
- April 1, 2002 – Feb. 28, 2006 : Assistant professor at Dept. of Chemical & Biomolecular Eng. KAIST
- Mar. 1, 2006 – present : Associate professor at Dept. of Chemical & Biomolecular Eng. KAIST
- Editorial Board Member, The Open Catalysis Journal (ISSN 1876-214X, Bentham Open), (2008~present)
- Editorial Board Member, Biotechnology Journal (ISSN 1860-6768, WILEY), (2010~present)
- Editorial Board Member, American Journal of Analytical Chemistry (Scientific Research Publishing), (2010~present)
- Nucleic acid bioengineering
- Microarray technology (DNA and cell chips)
- Electrochemical technology for molecular diagnostics
- Nanobiotechnology & enzyme engineering
1. Jae Yang Song, Hyun Gyu Park*, Sung-Ouk Jung and Jae Chan Park, “Diagnosis of HNF-1α mutations on a PNA zip- code microarray by single base extension”, Nucleic Acids Research, 33(2), e19-e26 (2005).
2. Yun Kyung Jung, Tae Won Kim, Jumi Kim, Jong-Man Kim* and Hyun Gyu Park* “Universal colorimetric detection of nucleic acids based on polydiacetylene (PDA) liposomes”, Advanced Functional Materials, 18(5), 701-708 (2008).
3. Yun Kyung Jung, Tae Won Kim, Cheulhee Jung, Dae-Yeon Cho and Hyun Gyu Park* “A polydiacetylene (PDA) microchip, based on a biotin-streptavidin (STA) interaction for the diagnosis of pathogen infections”, Small, 4(10), 1778-1784 (2008).
4. Hyo young Mun, Agnishwar Girigoswami, Cheulhee Jung, Dae-Yeon Cho, Hyun Gyu Park*, “SNPs detection by a single-strand specific nuclease on a PNA zip-code microarray”, Biosensors and Bioelectronics, 24(6), 1706-1711 (2009).
5. Byoung Yeon Won, Sung Chul Shin, Won-young Chung, Sujeong Shin, Dae-Yeon Cho and Hyun Gyu Park*, “Mismatch DNA-specific enzymatic cleavage employed in a new method for the electrochemical detection of genetic mutations”, Chemical Communications, (28), 4230-4232 (2009).
6. Rongzhan Fu, Taihua Li and Hyun Gyu Park*, “An ultrasensitive DNAzyme-based colorimetric strategy for nucleic acid detection”, Chemical Communications, (39), 5838-5840 (2009).
7. Moon Il Kim, Byung Jo Yu, Min-Ah Woo, Daeyeon Cho, Jonathan S. Dordick, June Hyoung Cho, Byung-Ok Choi and Hyun Gyu Park*, “Multiplexed amino acid array utilizing bioluminescent Escherichia coli auxotrophs”, Analytical Chemistry, 82(10), 4072-4077 (2010).
8. Taihua Li, Rongzhan Fu and Hyun Gyu Park*, “Pyrrolo-dC based fluorescent aptasensors for the molecular recognition of targets”, Chemical Communications, 46(19), 3271-3273 (2010).
9. Cheulhee Jung, Ji Won Chung, Un Ok Kim, Min Hwan Kim and Hyun Gyu Park*, “An isothermal target and probe amplification (iTPA) method, based on a combination of an isothermal chain amplification (ICA) technique and a FRET cycling probe technology (CPT)”, Analytical Chemistry, 82(14), 5937-5943 (2010).
10. Ki Soo Park, Cheulhee Jung, and Hyun Gyu Park*, “Illusionary polymerase activity triggered by metal ions: use for molecular logic-gate operations”, Angewandte Chemie International Edition, 49(50), 9757–9760 (2010). (Highlighted as an inside cover article)
Biochip & Biodevice Laboratory
Nucleic acid bioengineering, Biochips & Biosensor, Electrochemical diagnosis, Nanobiotechnology & Enzyme engineering
■ Nucleic acid bioengineering
1. Novel isothermal/quantitative nucleic acid amplification methods for ultra-high sensitive detection of biomolecules
The invention of the PCR technique has made a tremendous impact on the areas of biological research and diagnostics since it can be used to generate quantities of nucleic acids needed for detection and measurements. However, the need for a temperature cycling instrument limits applications of PCR in point-of-care testing (POCT) environments. In order to overcome this limitation, we have developed a powerful isothermal amplification method (ICA) as part of a quantitative amplification technology (iTPA) that combines ICA and FRET cycling probe technology (CPT). By creating a rationally designed amplification mechanism, ICA amplifies target DNA highly enough to detect down to 100 copies under isothermal conditions. To achieve the iTPA, dual amplification of both the target and the FRET probe is efficiently utilized to bring about high sensitivity. The observations made in this study clearly demonstrate that even a single copy level of C. trachomatis pathogen can be reliably quantified. Furthermore, combining a target and signaling probe amplification method (iTPA) with a colorimetric detection method (GCA), we have successfully developed a novel and efficient assay for the direct colorimetric diagnosis of C. trachomatis pathogen as low as 100 copies under isothermal conditions. The methods do not require an expensive thermo-cycler or other instrumentation and are remarkably simple and convenient. The proposed approaches are thus thought to be promising for application in portable sensor systems such as micro-fluidic devices or POCT diagnostic kits.
2. Metal ion aptamer-based target detection and logic gate
◈ The significance of this work derives from the following features.
☞ Being different from previous efforts in which interactions of nucleic acids with metal ions were studied, the current
investigation probed these interactions in combination with polymerase activity. The work has led to the discovery
that the illusionary activity of a polymerase can be intentionally triggered by using Hg2+ and Ag+ ions via their
interaction with the respective mismatched base pairs T-T and C-C. This phenomenon results in an unusual
polymerase amplification reaction.
☞ By utilizing this concept, a novel strategy to construct molecular scale logic gates was developed by rationally
designing primers and selecting the type of DNA polymerase employed. The novel strategy is both simple and cost
effective because it only requires incorporation of a single mismatched base (T and C) at the 3’ end of the primer and
the use of metal ions (Hg2+ and Ag+) as inputs. In contrast, previously reported strategies for the construction of logic
gates, which operate by specific metal ion regulation of the catalytic activity of deoxyribozymes, typically rely on
complicated designs for gate switching and frequently require expensive RNA or chimeric DNA as operational
substrates. These requirements significantly limit the utility of the older strategies.
Artificial DNAzymes (also called deoxyribozyme or catalytic DNA) are widely used as signal transduction components of biosensors because one DNAzyme molecule is able to catalyze many cycles of a specific reaction generating amplified readout signals. Among the DNAzymes acting as amplifying labels, a binary peroxidase DNAzyme attracted special efforts, which is composed of two single-stranded guanine-rich DNA strands self-assembling into G-quadruplex structure with hemin. Due to its ability to catalyze the generation of colorimetric or chemiluminescence signals, the binary peroxidase DNAzyme has been employed in many strategies for the detection of nucleic acids, small molecules, and proteins. Recently, we developed a novel ultrasensitive colorimetric detection method for nucleic acids. This method relies on a unique enzyme-mediated isothermal amplification strategy to realize triplex amplification effects on the readout signals: the target DNA recycling, the amplified generation of active DNAzymeMBs, and the DNAzyme-based signal amplification. This new isothermal colorimetric DNA detection method possesses ultra-high sensitivity with a detection limit down to 10 attomole range which was the lowest detection limit among the previous reports for peroxidase DNAzyme-based detection methods.
■ Microarray technology
■ Electrochemical technology
We are developing various electrochemical detection methods for genetic diagnosis. First, novel electrochemical strategies to detect nucleic acids were developed utilizing neutral charge property of peptide nucleic acid (PNA) or unique binding property of DNA intercalating agent. Second, a new detection method of DNA mutation or SNP genotyping was developed based on mismatched DNA-specific cleavage activity of CEL I endonuclease. These results developed by our group will serve as the basis for advanced electrochemical strategies for genetic diagnosis. In addition, we are currently developing the electrochemical real time PCR method.
2. Cell-based biosensor
3. Polydiacetylene (PDA)-based colorimetric biosensor