Department of Chemical and Biomolecular Engineering
Korea Advanced Institute of Science and Technology

Faculty

Yeu-Chun Kim (김유천)

Associate Professor

Tel : +82-42-350-3939
Fax : +82-42-350-3910
E-mail : dohnanyi@kaist.ac.kr
Homepage : http://bmnd.kaist.ac.kr
Education
- 2007 : Georgia Institute of Technology (Ph.D. in Chemical and Biomolecular Engineering)
- 2001 : KAIST (M. S. in Chemical Engineering)
- 1999 : Yonsei University (B.S. in Chemical Engineering)

Employment and Professional Experience
- 2012 ~ : Assistant Professor in Dept. Chemical and Biomolecular Engineering, KAIST
- 2011 : Senior Research Engineer, Korea Research Institute for Bioscience & Biotechnology (KRIBB)
- 2011 : Research Professor, Division of Nano Sciences, Ewha Womans University
- 2009 ~ 2010 : Visiting Scholar, Dept. Microbiology and Immunology, Emory University School of Medicine
- 2008 ~ 2010 : Postdoctoral Researcher, Dept. Chemical and Biomolecular Engineering, Georgia Institute of Technology
- 2000 ~ 2001 : Visiting Researcher, Korea Research Institute for Bioscience & Biotechnology (KRIBB)

Awards and Honors
- 2010 : Best Poster Award at the 1st International Conference on Microneedles
- 2009 : Edward Jenner Poster Award for the best poster presentation at the Vaccine 3rd Global Congress
- 2005 : Doh Won Suk Memorial Award of Korea Institute of Chemical Engineers-US Chapter

Research interests
- Biomedical device (Microneedle & Nanoneedle technology, Electroporation, Sonoporation, Iontophoresis)
- Drug delivery (Transdermal, Pumonary, Intranasal, Oral)
- Gene therapy (DNA vaccine delivery, siRNA delivery, gene correction)
- Biomass (Transformation of microalgae by gene delivery, Optimization of microalgae cultivation)
- Vaccine research (Effective vaccination, Mass production of recombinant vaccine, Vaccine adjuvant, Veterinary vaccine)
- Antimicrobial peptide research (Skin permeation enhancer, Gene delivery, Adjuvant)

Selected Publications
1. S.O.Choi, Y.C.Kim, J.W.Lee, J.H.Park, M.R.Prausnitz, M.G.Allen (2012) Intracellular protein delivery and gene transfection by electroporation using a microneedle electrode array, Small, Accepted.
2. Y.C.Kim, D.Zehrung, J.Courtney, S.Mitragotri, M.R.Prausnitz (2012) Delivery systems for intradermal vaccination, Current Topics in Microbiology and Immunology, 351 : 77-112
3. E.G.Gonzalez, Y.C.Kim, T.J.Speaker, R.P.Hickerson, R.Spitler, J.C.Birchall, M.F.Lara, R.Hu, Y.Liang, N.K.Smith, M.R.Prausnitz, C.H.Contag, L.M.Milstone, R.L.Kaspar (2011) Visualization of plasmid delivery to keratinocytes in mouse and human epidermis, Scientific Reports, 1:158.
4. Y.C.Kim, F.S.Quan, R.W.Compans, S.M.Kang, M.R.Prausnitz (2011) Long-term storage and stability kinetics of influenza vaccines using coated microneedle, Pharmaceutical Research, 28 : 135-144.
5. F.S.Quan, Y.C.Kim*, R.W.Compans, M.R.Prausnitz, S.M.Kang (2010) Dose sparing microneedles vaccination with H1 influenza VLPs, Journal of Controlled Release, 147 : 326-332.
6. F.S.Quan, Y.C.Kim*, A. Vunnava, D.G.Yoo, J.M.Song, M.R.Prausnitz, R.W.Compans, S.M.Kang (2010) Intradermal vaccination with influenza VLPs using microneedles induces superior protection to intramuscular immunization, Journal of Virology 84 : 7760-7769.
7. S.O.Choi, Y.C.Kim, J.H.Park, J.Hutcheson, H.S. Gill, Y.-K. Yoon, M.R. Prausnitz, and M.G. Allen (2010) An electrically active microneedle array for electroporation, Biomedical Microdevices 12 : 263-273.
8. Y.C.Kim, F.S.Quan, D.G.Yoo, R.W.Compans, S.M.Kang, M.R.Prausnitz (2010) Enhanced memory responses to H1N1 influenza vaccinationin the skin using vaccine coated-microneedles, Journal of Infectious Disease 201 : 190-198.
9. Y.C.Kim, F.S.Quan, R.W.Compans, S.M.Kang, M.R.Prausnitz (2010) Formulation and coating of microneedles with inactivated influenza virus to improve vaccine stability and immunogenicity, Journal of Controlled Release 142 : 187-195.
10. F.S.Quan, Y.C.Kim*, D.G.Yoo, R.W.Compans, M.R.Prausnitz, S.M.Kang (2009) Stabilization of influenza vaccine enhances protection by microneedle delivery in the skin, Plos ONE 4 : e7152.
11. Y.C.Kim, F.S.Quan, D.G.Yoo, R.W.Compans, S.M.Kang, M.R.Prausnitz (2009) Improved influenza vaccination in the skin using vaccine coated-microneedles, Vaccine 27 : 6932-6938.
12. Y.C.Kim, S.Late, A.Banga, P.Ludovice, M.Prausnitz (2008) Biochemical enhancement of transdermal delivery with magainin peptide: Modification of electrostatic interactions by changing pH, International Journal of Pharmaceutics 362 : 20-28.
13. Y.C.Kim, J.H.Park, P.Ludovice, M.Prausnitz (2008) Synergistic enhancement of skin permeability by N-lauroylsarcosine and ethanol, International Journal of Pharmaceutics 352 : 129–138.
14. Y.C.Kim, P.Ludovice, M.Prausnitz (2007) Transdermal delivery enhanced by magainin pore-forming peptide, Journal of Controlled Release 22 : 375-383.
* Equally contribution. Co-first author.

Biomedical Micro-Nano Delivery Laboratory
Biomedical device, Drug delivery, Gene therapy, Biomass, Vaccine research, Antimicrobial peptide

 

■ Biomedical device

 

A biomedical device is a tool used for medical purposes such as diagnosis and therapy. There are numerous biomedical devices to improve human health and welfare by altering pharmacological, metabolic, or immunological functions. To achieve fast, economic, non-invasive, and sensitive biomedical applications like diagnostics, treatment, and drug delivery, development of smaller scale medical device is demanding. We are interested in development and application of micro- or nano-scale biomedical devices such as micro-or nano-needles and various types of micro- or nano-implants.


■ Drug delivery


Drug delivery is the modulation of pharmaceutical or biopharmaceutical administration for efficient therapeutic effect. In order to improve the efficacy, safety, and patient compliance of drugs, drug delivery researches have focused on how to reduce toxicity, increase absorption and distribution, and improve release profile. Various routes of delivery including oral, pulmonary, intranasal, intravaginal, and transdermal have been investigated, and we delve into advanced transdermal drug delivery.


■ Gene therapy


Genes are the basic molecular units of heredity of a living organism and achieve their functions by directing protein synthesis. Gene therapy is a technique for inserting, altering, or removing genes within cells and tissues. Generally, a normal gene is inserted into the genome to replace a disease-causing gene. The viral vector is considered the most common method for effective gene therapy, but it has safety issues. We are working on non-viral gene therapy including various physical methods such as electroporation, micro-or nano-particle carriers, and micro- or nano-needle gene delivery.


■ Biomass


Due to upcoming energy crisis which results from a disruption of energy supplies and limitation of fossil fuels, biomass has been considered as a promising alternative for energy source. Biomass can be converted into other energy products such as biofuel and microalgae have been studied intensively as a critical candidate for biomass resources.  Using our delivery technique, we can transform and screen best-performing strain of microalgae by inserting gene using micro-or nanoneedle or microelectroporation.


■ Vaccine research


Vaccination is the most effective way to prevent the spread of infectious disease. The recent outbreak of pandemic H1N1 influenza gave us a lesson how vaccines are critical for global health. However, current vaccine production systems and administration methods have several limitations. We focus on two aspects of vaccine research: optimization of recombinant vaccine production by genetic engineering and development of effective vaccine administration methods such as skin vaccination.


■ Antimicrobial peptide research


Antimicrobial peptides have been shown to kill microbes with impressive potency and specificity and developed as therapeutic agents for infectious disease treatment. Permeating peptides can also be applied as carriers for delivery of foreign substances into microorganisms. We study the novel application of antimicrobial peptide as a skin permeation enhancer and vaccine adjuvant. The mechanism of permeation enhancement and the adjuvant effects of antimicrobial peptides will be elucidated by various biological assays.